Development of a rapid isolation procedure for coccolith ultrafine particles produced by coccolithophorid algae

Hiroyuki Takano, Eichi Manabe, Morio Hirano, Megumi Okazaki, J. Grant Burgess, Noriyuki Nakamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A rapid procedure for effective purification of large quantities of coccolith ultrafine particles from marine algae is reported. Coccoliths are detached from cells by optimized sonication in the presence of 50 m M NaHCO3. Contaminating cell debris is then removed from coccoliths by cycles of washing and floatation. Coccolith particles were purified from Emiliania huxleyi and Pleurochrysis carterae. The surface area of these particles is three to five times greater than synthetic calcite particles. Glucose oxidase and uricase have been immobilized onto purified coccolith ultrafine particles to illustrate their potential as a support material for biotechnological application.

Original languageEnglish
Pages (from-to)239-247
Number of pages9
JournalApplied Biochemistry and Biotechnology
Volume39-40
Issue number1
DOIs
Publication statusPublished - 1993 Sep
Externally publishedYes

Fingerprint

Haptophyta
Glucose oxidase
Sonication
Calcite
Algae
Debris
Washing
Purification
Urate Oxidase
Glucose Oxidase
Calcium Carbonate
Ultrafine

Keywords

  • calcite ultrafine particles
  • coccolith
  • Coccolithophorid algae
  • enzyme immobilization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Development of a rapid isolation procedure for coccolith ultrafine particles produced by coccolithophorid algae. / Takano, Hiroyuki; Manabe, Eichi; Hirano, Morio; Okazaki, Megumi; Burgess, J. Grant; Nakamura, Noriyuki; Matsunaga, Tadashi.

In: Applied Biochemistry and Biotechnology, Vol. 39-40, No. 1, 09.1993, p. 239-247.

Research output: Contribution to journalArticle

Takano, Hiroyuki ; Manabe, Eichi ; Hirano, Morio ; Okazaki, Megumi ; Burgess, J. Grant ; Nakamura, Noriyuki ; Matsunaga, Tadashi. / Development of a rapid isolation procedure for coccolith ultrafine particles produced by coccolithophorid algae. In: Applied Biochemistry and Biotechnology. 1993 ; Vol. 39-40, No. 1. pp. 239-247.
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