Development of a ubiquitously transferrable silver-nanoparticle-loaded polymer nanosheet as an antimicrobial coating

Keisuke Ito, Akihiro Saito, Toshinori Fujie, Hiromi Miyazaki, Manabu Kinoshita, Daizoh Saitoh, Shinya Ohtsubo, Shinji Takeoka

Research output: Contribution to journalArticle

11 Citations (Scopus)


Ultra-thin polymer films (nanosheets) fabricated by a layer-by-layer (LbL) method possess unique properties such as high flexibility, adhesive strength, and transparency, and can be peeled off from a substrate and attached to various surfaces via a water-soluble supporting film. Therefore, flexible and transferrable LbL nanosheets are convenient tools as coating materials. Here, we fabricated a novel antimicrobial coating material by embedding silver nanoparticles (AgNPs) in an LbL nanosheet composed of layers of chitosan and sodium alginate (Ag-LbL nanosheet) by means of a photo-reduction method. Optimizing the amount of irradiated energy applied led to robust antimicrobial efficacy against methicillin-resistant Staphylococcus aureus (MRSA), sufficient to meet ISO standards (ISO 22196), while maintaining the flexibility and adhesive potency of the LbL nanosheet. Thus, the Ag-LbL nanosheet is a promising coating material that can provide antimicrobial efficacy to various surfaces.

Original languageEnglish
Pages (from-to)585-593
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number3
Publication statusPublished - 2016 Apr 1



  • antimicrobial
  • bacterial adherence
  • coating
  • in vitro
  • polymer

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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