Development of an early estimation method for predicting later osteogenic differentiation activity of rat mesenchymal stromal cells from their attachment areas

Kan Cheng, Motohiro Hirose, Xiupeng Wang, Yu Sogo, Atsushi Yamazaki, Atsuo Ito

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Cell morphology has received considerable attention in recent years owing to its possible relationship with cell functions, including proliferation, differentiation, and migration. Recent evidence suggests that extracellular environments can also mediate cell functions, particularly cell adhesion. The aims of this study were to investigate the correlation between osteogenic differentiation activity and the morphology of rat mesenchymal stromal cells (MSCs), and to develop a method of estimating osteogenic differentiation capability of MSCs on biomaterials. We measured the attachment areas of MSCs on substrates with various types of surface after 2 h of seeding, and quantified the amount of osteocalcin secreted from MSCs after 3 weeks of culture under osteogenic differentiation conditions. MSCs with small attachment areas showed a high osteogenic differentiation activity. These findings indicate that cell attachment areas correlate well with the osteogenic differentiation activity of MSCs. They also suggest that the measurement of cell attachment areas is useful for estimating the osteogenic differentiation activity of MSCs and is a practical tool for applications of MSCs in regenerative medicine.

    Original languageEnglish
    Article number064209
    JournalScience and Technology of Advanced Materials
    Volume13
    Issue number6
    DOIs
    Publication statusPublished - 2012 Dec

    Fingerprint

    Rats
    Osteocalcin
    Cell adhesion
    Biocompatible Materials
    Biomaterials
    Substrates
    Regenerative Medicine

    Keywords

    • apatite
    • cell morphology
    • mesenchymal stromal cells
    • osteogenic differentiation activity
    • proliferative activity
    • regenerative medicine
    • titanium

    ASJC Scopus subject areas

    • Materials Science(all)

    Cite this

    Development of an early estimation method for predicting later osteogenic differentiation activity of rat mesenchymal stromal cells from their attachment areas. / Cheng, Kan; Hirose, Motohiro; Wang, Xiupeng; Sogo, Yu; Yamazaki, Atsushi; Ito, Atsuo.

    In: Science and Technology of Advanced Materials, Vol. 13, No. 6, 064209, 12.2012.

    Research output: Contribution to journalArticle

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