Development of automated high throughput single molecular microfluidic detection platform for signal transduction analysis

Po Jung Huang, Sina Baghbani Kordmahale, Chao Kai Chou, Hirohito Yamaguchi, Mien Chie Hung, Jun Kameoka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Signal transductions including multiple protein post-translational modifications (PTM), protein-protein interactions (PPI), and protein-nucleic acid interaction (PNI) play critical roles for cell proliferation and differentiation that are directly related to the cancer biology. Traditional methods, like mass spectrometry, immunoprecipitation, fluorescence resonance energy transfer, and fluorescence correlation spectroscopy require a large amount of sample and long processing time. "microchannel for multiple-parameter analysis of proteins in single-complex (mMAPS)"we proposed can reduce the process time and sample volume because this system is composed by microfluidic channels, fluorescence microscopy, and computerized data analysis. In this paper, we will present an automated mMAPS including integrated microfluidic device, automated stage and electrical relay for high-throughput clinical screening. Based on this result, we estimated that this automated detection system will be able to screen approximately 150 patient samples in a 24-hour period, providing a practical application to analyze tissue samples in a clinical setting.

Original languageEnglish
Title of host publicationMicrofluidics, BioMEMS, and Medical Microsystems XIV
EditorsHolger Becker, Bonnie L. Gray
PublisherSPIE
ISBN (Electronic)9781628419399
DOIs
Publication statusPublished - 2016
Externally publishedYes
Event14th SPIE Photonics West Conference: Microfluidics, BioMEMS, and Medical Microsystems - San Francisco, United States
Duration: 2016 Feb 132016 Feb 15

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9705
ISSN (Print)1605-7422

Conference

Conference14th SPIE Photonics West Conference: Microfluidics, BioMEMS, and Medical Microsystems
Country/TerritoryUnited States
CitySan Francisco
Period16/2/1316/2/15

Keywords

  • automated system
  • flow-proteomic
  • high-throughput analysis
  • Microfluidic device
  • protein-protein complex interaction
  • single molecule detection

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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