Development of efficient expression system for protein display on bacterial magnetic particles

Tomoko Yoshino, Tadashi Matsunaga

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Bacterial magnetic particles (BMPs) are utilized for various biomedical applications because they are easily manipulated by magnets, and functional proteins are easily displayed on BMPs. To establish highly expressed protein display on BMPs, strong promoters were identified using Magnetospirillum magneticum AMB-1 genome and proteome databases. Initially, several proteins highly expressed in AMB-1 were identified, and the upstream DNA sequences of the open-reading frames were evaluated using a luciferase-reporter gene assay to compare promoter activities. Consequently, luminescence intensity was 400 times higher due to the novel promoter identified in this study than the magA promoter previously used. Subsequently, efficient protein display on BMPs was performed using the newly identified promoter sequences. This developed display system will facilitate the assembly of various functional proteins onto BMPs to create novel magnetic nanoparticles.

Original languageEnglish
Pages (from-to)1678-1681
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume338
Issue number4
DOIs
Publication statusPublished - 2005 Dec 30
Externally publishedYes

Fingerprint

Display devices
Magnetospirillum
Proteins
Genes
Bacterial Proteins
Magnets
DNA sequences
Proteome
Luminescence
Luciferases
Reporter Genes
Nanoparticles
Open Reading Frames
Assays
Genome
Databases

Keywords

  • Bacterial magnetic particles
  • Luciferase
  • Magnetospirillum magneticum AMB-1
  • Promoter
  • Protein display

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Development of efficient expression system for protein display on bacterial magnetic particles. / Yoshino, Tomoko; Matsunaga, Tadashi.

In: Biochemical and Biophysical Research Communications, Vol. 338, No. 4, 30.12.2005, p. 1678-1681.

Research output: Contribution to journalArticle

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