Abstract
Bacterial magnetic particles (BMPs) are utilized for various biomedical applications because they are easily manipulated by magnets, and functional proteins are easily displayed on BMPs. To establish highly expressed protein display on BMPs, strong promoters were identified using Magnetospirillum magneticum AMB-1 genome and proteome databases. Initially, several proteins highly expressed in AMB-1 were identified, and the upstream DNA sequences of the open-reading frames were evaluated using a luciferase-reporter gene assay to compare promoter activities. Consequently, luminescence intensity was 400 times higher due to the novel promoter identified in this study than the magA promoter previously used. Subsequently, efficient protein display on BMPs was performed using the newly identified promoter sequences. This developed display system will facilitate the assembly of various functional proteins onto BMPs to create novel magnetic nanoparticles.
| Original language | English |
|---|---|
| Pages (from-to) | 1678-1681 |
| Number of pages | 4 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 338 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2005 Dec 30 |
| Externally published | Yes |
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Keywords
- Bacterial magnetic particles
- Luciferase
- Magnetospirillum magneticum AMB-1
- Promoter
- Protein display
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Development of efficient expression system for protein display on bacterial magnetic particles. / Yoshino, Tomoko; Matsunaga, Tadashi.
In: Biochemical and Biophysical Research Communications, Vol. 338, No. 4, 30.12.2005, p. 1678-1681.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of efficient expression system for protein display on bacterial magnetic particles
AU - Yoshino, Tomoko
AU - Matsunaga, Tadashi
PY - 2005/12/30
Y1 - 2005/12/30
N2 - Bacterial magnetic particles (BMPs) are utilized for various biomedical applications because they are easily manipulated by magnets, and functional proteins are easily displayed on BMPs. To establish highly expressed protein display on BMPs, strong promoters were identified using Magnetospirillum magneticum AMB-1 genome and proteome databases. Initially, several proteins highly expressed in AMB-1 were identified, and the upstream DNA sequences of the open-reading frames were evaluated using a luciferase-reporter gene assay to compare promoter activities. Consequently, luminescence intensity was 400 times higher due to the novel promoter identified in this study than the magA promoter previously used. Subsequently, efficient protein display on BMPs was performed using the newly identified promoter sequences. This developed display system will facilitate the assembly of various functional proteins onto BMPs to create novel magnetic nanoparticles.
AB - Bacterial magnetic particles (BMPs) are utilized for various biomedical applications because they are easily manipulated by magnets, and functional proteins are easily displayed on BMPs. To establish highly expressed protein display on BMPs, strong promoters were identified using Magnetospirillum magneticum AMB-1 genome and proteome databases. Initially, several proteins highly expressed in AMB-1 were identified, and the upstream DNA sequences of the open-reading frames were evaluated using a luciferase-reporter gene assay to compare promoter activities. Consequently, luminescence intensity was 400 times higher due to the novel promoter identified in this study than the magA promoter previously used. Subsequently, efficient protein display on BMPs was performed using the newly identified promoter sequences. This developed display system will facilitate the assembly of various functional proteins onto BMPs to create novel magnetic nanoparticles.
KW - Bacterial magnetic particles
KW - Luciferase
KW - Magnetospirillum magneticum AMB-1
KW - Promoter
KW - Protein display
UR - http://www.scopus.com/inward/record.url?scp=27844446643&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27844446643&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.10.148
DO - 10.1016/j.bbrc.2005.10.148
M3 - Article
C2 - 16288989
AN - SCOPUS:27844446643
VL - 338
SP - 1678
EP - 1681
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -