Development of Erythroid Progenitors under Erythropoietin Stimulation in Xenopus laevis Larval Liver

Takehito Okui, Sakiko Hosozawa, Satoka Kohama, Shingo Fujiyama, Shun Maekawa, Hiroshi Muto, Takashi Kato

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    2 Citations (Scopus)

    Abstract

    Erythroid progenitors that respond to erythropoietin (Epo) are present in the liver of adult Xenopus laevis. However, cells responding to Epo in the larval liver and through the metamorphosis period under hepatic remodeling have not been characterized. In this study, tadpoles were staged using the tables of Nieuwkoop and Faber (NF). Liver cells from pre-(NF56) or post-(NF66) metamorphic stage were cultured in the presence of Epo. β2-globin mRNA expression peaked at day 7 after the start of culture. Larval β2-globin was highly expressed in NF56-derived cells, while adult β2-globinwas detected in those of NF66. In both NF56-and NF66-derived cells, mRNA expression of eporand gata2 peaked at day 5 and days 3-4, respectively. In contrast, gata1 expression peaked at day 6 in NF56 cells and at day 5 in NF66 cells. Half maximal proliferation of erythrocytic blast cells derived from the liver at NF66 was observed at day 3, which was earlier than that of NF56. These results indicate that erythroid progenitors that respond to Xenopus laevis Epo are maintained in pre-and post-metamorphic liver, although the tissue architecture changes dramatically during metamorphosis. Additionally, the globin switching occurred, and/or the erythroid progenitors for larval erythrocytes were replaced by those for adult erythrocytes in the metamorphic liver.

    Original languageEnglish
    Pages (from-to)575-582
    Number of pages8
    JournalZoological Science
    Volume33
    Issue number6
    DOIs
    Publication statusPublished - 2016 Dec 1

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    Keywords

    • African clawed frog
    • erythropoiesis
    • erythropoietin
    • hematopoiesis
    • liver
    • metamorphosis
    • tadpole

    ASJC Scopus subject areas

    • Animal Science and Zoology

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