Development of Flexible Cell-Loaded Ultrathin Ribbons for Minimally Invasive Delivery of Skeletal Muscle Cells

Sahar Salehi, Serge Ostrovidov, Majid Ebrahimi, Ramin Banan Sadeghian, Xiaobin Liang, Ken Nakajima, Hojae Bae, Toshinori Fujie, Ali Khademhosseini

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cell transplantation therapy provides a potential solution for treating skeletal muscle disorders, but cell survival after transplantation is poor. This limitation could be addressed by grafting donor cells onto biomaterials to protect them against harsh environments and processing, consequently improving cell viability in situ. Thus, we present here the fabrication of poly(lactic-co-glycolic acid) (PLGA) ultrathin ribbons with "canal-like" structures using a microfabrication technique to generate ribbons of aligned murine skeletal myoblasts (C2C12). We found that the ribbons functionalized with a solution of 3,4-dihydroxy-l-phenylalanine (DOPA) and then coated with poly-l-lysine (PLL) and fibronectin (FN) improve cell attachment and support the growth of C2C12. The viability of cells on the ribbons is evaluated following the syringe-handling steps of injection with different needle sizes. C2C12 cells readily adhere to the ribbon surface, proliferate over time, align (over 74%), maintain high viability (over 80%), and differentiate to myotubes longer than 400 μm. DNA content quantification carried out before and after injection and myogenesis evaluation confirm that cell-loaded ribbons can safely retain cells with high functionality after injection and are suitable for minimally invasive cell transplantation.

Original languageEnglish
Pages (from-to)579-589
Number of pages11
JournalACS Biomaterials Science and Engineering
Volume3
Issue number4
DOIs
Publication statusPublished - 2017 Apr 10

Fingerprint

Muscle
Cells
Syringes
Microfabrication
Biocompatible Materials
Canals
Phenylalanine
Fibronectins
Biomaterials
Needles
Lysine
DNA
Fabrication
Acids
Processing
polylactic acid-polyglycolic acid copolymer
Milk

Keywords

  • immobilization
  • injectable materials
  • microfabrication
  • skeletal muscle cells
  • tissue engineering
  • ultrathin ribbons

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Salehi, S., Ostrovidov, S., Ebrahimi, M., Sadeghian, R. B., Liang, X., Nakajima, K., ... Khademhosseini, A. (2017). Development of Flexible Cell-Loaded Ultrathin Ribbons for Minimally Invasive Delivery of Skeletal Muscle Cells. ACS Biomaterials Science and Engineering, 3(4), 579-589. https://doi.org/10.1021/acsbiomaterials.6b00696

Development of Flexible Cell-Loaded Ultrathin Ribbons for Minimally Invasive Delivery of Skeletal Muscle Cells. / Salehi, Sahar; Ostrovidov, Serge; Ebrahimi, Majid; Sadeghian, Ramin Banan; Liang, Xiaobin; Nakajima, Ken; Bae, Hojae; Fujie, Toshinori; Khademhosseini, Ali.

In: ACS Biomaterials Science and Engineering, Vol. 3, No. 4, 10.04.2017, p. 579-589.

Research output: Contribution to journalArticle

Salehi, S, Ostrovidov, S, Ebrahimi, M, Sadeghian, RB, Liang, X, Nakajima, K, Bae, H, Fujie, T & Khademhosseini, A 2017, 'Development of Flexible Cell-Loaded Ultrathin Ribbons for Minimally Invasive Delivery of Skeletal Muscle Cells', ACS Biomaterials Science and Engineering, vol. 3, no. 4, pp. 579-589. https://doi.org/10.1021/acsbiomaterials.6b00696
Salehi, Sahar ; Ostrovidov, Serge ; Ebrahimi, Majid ; Sadeghian, Ramin Banan ; Liang, Xiaobin ; Nakajima, Ken ; Bae, Hojae ; Fujie, Toshinori ; Khademhosseini, Ali. / Development of Flexible Cell-Loaded Ultrathin Ribbons for Minimally Invasive Delivery of Skeletal Muscle Cells. In: ACS Biomaterials Science and Engineering. 2017 ; Vol. 3, No. 4. pp. 579-589.
@article{a220e505eb2b4150aa3de3978c70af2a,
title = "Development of Flexible Cell-Loaded Ultrathin Ribbons for Minimally Invasive Delivery of Skeletal Muscle Cells",
abstract = "Cell transplantation therapy provides a potential solution for treating skeletal muscle disorders, but cell survival after transplantation is poor. This limitation could be addressed by grafting donor cells onto biomaterials to protect them against harsh environments and processing, consequently improving cell viability in situ. Thus, we present here the fabrication of poly(lactic-co-glycolic acid) (PLGA) ultrathin ribbons with {"}canal-like{"} structures using a microfabrication technique to generate ribbons of aligned murine skeletal myoblasts (C2C12). We found that the ribbons functionalized with a solution of 3,4-dihydroxy-l-phenylalanine (DOPA) and then coated with poly-l-lysine (PLL) and fibronectin (FN) improve cell attachment and support the growth of C2C12. The viability of cells on the ribbons is evaluated following the syringe-handling steps of injection with different needle sizes. C2C12 cells readily adhere to the ribbon surface, proliferate over time, align (over 74{\%}), maintain high viability (over 80{\%}), and differentiate to myotubes longer than 400 μm. DNA content quantification carried out before and after injection and myogenesis evaluation confirm that cell-loaded ribbons can safely retain cells with high functionality after injection and are suitable for minimally invasive cell transplantation.",
keywords = "immobilization, injectable materials, microfabrication, skeletal muscle cells, tissue engineering, ultrathin ribbons",
author = "Sahar Salehi and Serge Ostrovidov and Majid Ebrahimi and Sadeghian, {Ramin Banan} and Xiaobin Liang and Ken Nakajima and Hojae Bae and Toshinori Fujie and Ali Khademhosseini",
year = "2017",
month = "4",
day = "10",
doi = "10.1021/acsbiomaterials.6b00696",
language = "English",
volume = "3",
pages = "579--589",
journal = "ACS Biomaterials Science and Engineering",
issn = "2373-9878",
publisher = "American Chemical Society",
number = "4",

}

TY - JOUR

T1 - Development of Flexible Cell-Loaded Ultrathin Ribbons for Minimally Invasive Delivery of Skeletal Muscle Cells

AU - Salehi, Sahar

AU - Ostrovidov, Serge

AU - Ebrahimi, Majid

AU - Sadeghian, Ramin Banan

AU - Liang, Xiaobin

AU - Nakajima, Ken

AU - Bae, Hojae

AU - Fujie, Toshinori

AU - Khademhosseini, Ali

PY - 2017/4/10

Y1 - 2017/4/10

N2 - Cell transplantation therapy provides a potential solution for treating skeletal muscle disorders, but cell survival after transplantation is poor. This limitation could be addressed by grafting donor cells onto biomaterials to protect them against harsh environments and processing, consequently improving cell viability in situ. Thus, we present here the fabrication of poly(lactic-co-glycolic acid) (PLGA) ultrathin ribbons with "canal-like" structures using a microfabrication technique to generate ribbons of aligned murine skeletal myoblasts (C2C12). We found that the ribbons functionalized with a solution of 3,4-dihydroxy-l-phenylalanine (DOPA) and then coated with poly-l-lysine (PLL) and fibronectin (FN) improve cell attachment and support the growth of C2C12. The viability of cells on the ribbons is evaluated following the syringe-handling steps of injection with different needle sizes. C2C12 cells readily adhere to the ribbon surface, proliferate over time, align (over 74%), maintain high viability (over 80%), and differentiate to myotubes longer than 400 μm. DNA content quantification carried out before and after injection and myogenesis evaluation confirm that cell-loaded ribbons can safely retain cells with high functionality after injection and are suitable for minimally invasive cell transplantation.

AB - Cell transplantation therapy provides a potential solution for treating skeletal muscle disorders, but cell survival after transplantation is poor. This limitation could be addressed by grafting donor cells onto biomaterials to protect them against harsh environments and processing, consequently improving cell viability in situ. Thus, we present here the fabrication of poly(lactic-co-glycolic acid) (PLGA) ultrathin ribbons with "canal-like" structures using a microfabrication technique to generate ribbons of aligned murine skeletal myoblasts (C2C12). We found that the ribbons functionalized with a solution of 3,4-dihydroxy-l-phenylalanine (DOPA) and then coated with poly-l-lysine (PLL) and fibronectin (FN) improve cell attachment and support the growth of C2C12. The viability of cells on the ribbons is evaluated following the syringe-handling steps of injection with different needle sizes. C2C12 cells readily adhere to the ribbon surface, proliferate over time, align (over 74%), maintain high viability (over 80%), and differentiate to myotubes longer than 400 μm. DNA content quantification carried out before and after injection and myogenesis evaluation confirm that cell-loaded ribbons can safely retain cells with high functionality after injection and are suitable for minimally invasive cell transplantation.

KW - immobilization

KW - injectable materials

KW - microfabrication

KW - skeletal muscle cells

KW - tissue engineering

KW - ultrathin ribbons

UR - http://www.scopus.com/inward/record.url?scp=85017551299&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85017551299&partnerID=8YFLogxK

U2 - 10.1021/acsbiomaterials.6b00696

DO - 10.1021/acsbiomaterials.6b00696

M3 - Article

AN - SCOPUS:85017551299

VL - 3

SP - 579

EP - 589

JO - ACS Biomaterials Science and Engineering

JF - ACS Biomaterials Science and Engineering

SN - 2373-9878

IS - 4

ER -