Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system

Reiko Sato, Haruko Takeyama, Tsuyoshi Tanaka, Tadashi Matsunaga

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.

Original languageEnglish
Pages (from-to)109-116
Number of pages8
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume91-93
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Bacterial Antibodies
Allergens
Muramidase
Immunoassay
Antibodies
Enzymes
Food
Egg White
Chemiluminescence
Magnets
Luminescence
Limit of Detection
Assays

Keywords

  • Bacterial magnetic particles
  • Food allergen
  • Fully automated system
  • Lysozyme
  • Sandwich immunoassay

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

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abstract = "A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10{\%} coefficient of variation.",
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AU - Tanaka, Tsuyoshi

AU - Matsunaga, Tadashi

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