Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system

Reiko Sato, Haruko Takeyama, Tsuyoshi Tanaka, Tadashi Matsunaga

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.

Original languageEnglish
Pages (from-to)109-116
Number of pages8
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume91-93
DOIs
Publication statusPublished - 2001 May 23
Externally publishedYes

Keywords

  • Bacterial magnetic particles
  • Food allergen
  • Fully automated system
  • Lysozyme
  • Sandwich immunoassay

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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