Abstract
A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.
| Original language | English |
|---|---|
| Pages (from-to) | 109-116 |
| Number of pages | 8 |
| Journal | Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology |
| Volume | 91-93 |
| DOIs | |
| Publication status | Published - 2001 |
| Externally published | Yes |
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Keywords
- Bacterial magnetic particles
- Food allergen
- Fully automated system
- Lysozyme
- Sandwich immunoassay
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
- Bioengineering
Cite this
Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system. / Sato, Reiko; Takeyama, Haruko; Tanaka, Tsuyoshi; Matsunaga, Tadashi.
In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 91-93, 2001, p. 109-116.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system
AU - Sato, Reiko
AU - Takeyama, Haruko
AU - Tanaka, Tsuyoshi
AU - Matsunaga, Tadashi
PY - 2001
Y1 - 2001
N2 - A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.
AB - A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.
KW - Bacterial magnetic particles
KW - Food allergen
KW - Fully automated system
KW - Lysozyme
KW - Sandwich immunoassay
UR - http://www.scopus.com/inward/record.url?scp=0035041512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035041512&partnerID=8YFLogxK
U2 - 10.1385/ABAB:91-93:1-9:109
DO - 10.1385/ABAB:91-93:1-9:109
M3 - Article
C2 - 11963840
AN - SCOPUS:0035041512
VL - 91-93
SP - 109
EP - 116
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
SN - 0273-2289
ER -