Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system

Reiko Sato; Haruko Takeyama; Tsuyoshi Tanaka; Tadashi Matsunaga

doi:10.1385/ABAB:91-93:1-9:109

Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system

Reiko Sato, Haruko Takeyama, Tsuyoshi Tanaka, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.

Original language	English
Pages (from-to)	109-116
Number of pages	8
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	91-93
DOIs	https://doi.org/10.1385/ABAB:91-93:1-9:109
Publication status	Published - 2001 May 23
Externally published	Yes

Keywords

Bacterial magnetic particles
Food allergen
Fully automated system
Lysozyme
Sandwich immunoassay

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology

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10.1385/ABAB:91-93:1-9:109

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Sato, R., Takeyama, H., Tanaka, T., & Matsunaga, T. (2001). Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system. Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, 91-93, 109-116. https://doi.org/10.1385/ABAB:91-93:1-9:109

Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system. / Sato, Reiko; Takeyama, Haruko; Tanaka, Tsuyoshi et al.

In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 91-93, 23.05.2001, p. 109-116.

Research output: Contribution to journal › Article › peer-review

Sato, R, Takeyama, H, Tanaka, T & Matsunaga, T 2001, 'Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system', Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, vol. 91-93, pp. 109-116. https://doi.org/10.1385/ABAB:91-93:1-9:109

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Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system

Abstract

Keywords

ASJC Scopus subject areas

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