Development of high-sensitive cytotoxic detection system using HSP70B promoter

K. I. Wada, L. Xu, T. Okano, A. Taniguchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The development of a high-sensitive cytotoxic detection system using heat-shock proteins (HSP)70B promoter was discussed. Human liver carcinoma cell line was grown in DMEM at 37 °C in a humidified atmosphere with 5% CO 2. Cell viability was evaluated by quantification of DNA performed by using Cyquant Proliferation Assay kit. Subsequent sequencing revealed that transcriptional starting site was mapped at - 110 bp from the translational starting site in HepG2. These results suggests that the effect of low concentration of cadmium chloride on cytotoxicity could be detected by luciferase analysis using a part of HASP70B promoter.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
Publication statusPublished - 2004 Dec 1
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 2004 May 172004 May 21

Publication series

NameTransactions - 7th World Biomaterials Congress

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period04/5/1704/5/21

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Wada, K. I., Xu, L., Okano, T., & Taniguchi, A. (2004). Development of high-sensitive cytotoxic detection system using HSP70B promoter. In Transactions - 7th World Biomaterials Congress (Transactions - 7th World Biomaterials Congress).