Development of high-sensitive cytotoxic detection system using HSP70B promoter

K. I. Wada, L. Xu, T. Okano, Akiyoshi Taniguchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The development of a high-sensitive cytotoxic detection system using heat-shock proteins (HSP)70B promoter was discussed. Human liver carcinoma cell line was grown in DMEM at 37 °C in a humidified atmosphere with 5% CO 2. Cell viability was evaluated by quantification of DNA performed by using Cyquant Proliferation Assay kit. Subsequent sequencing revealed that transcriptional starting site was mapped at - 110 bp from the translational starting site in HepG2. These results suggests that the effect of low concentration of cadmium chloride on cytotoxicity could be detected by luciferase analysis using a part of HASP70B promoter.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Pages1248
Number of pages1
Publication statusPublished - 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 2004 May 172004 May 21

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period04/5/1704/5/21

Fingerprint

Cells
Cadmium chloride
Cytotoxicity
Liver
Assays
DNA
Proteins
Hot Temperature

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Wada, K. I., Xu, L., Okano, T., & Taniguchi, A. (2004). Development of high-sensitive cytotoxic detection system using HSP70B promoter. In Transactions - 7th World Biomaterials Congress (pp. 1248)

Development of high-sensitive cytotoxic detection system using HSP70B promoter. / Wada, K. I.; Xu, L.; Okano, T.; Taniguchi, Akiyoshi.

Transactions - 7th World Biomaterials Congress. 2004. p. 1248.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wada, KI, Xu, L, Okano, T & Taniguchi, A 2004, Development of high-sensitive cytotoxic detection system using HSP70B promoter. in Transactions - 7th World Biomaterials Congress. pp. 1248, Transactions - 7th World Biomaterials Congress, Sydney, Australia, 04/5/17.
Wada KI, Xu L, Okano T, Taniguchi A. Development of high-sensitive cytotoxic detection system using HSP70B promoter. In Transactions - 7th World Biomaterials Congress. 2004. p. 1248
Wada, K. I. ; Xu, L. ; Okano, T. ; Taniguchi, Akiyoshi. / Development of high-sensitive cytotoxic detection system using HSP70B promoter. Transactions - 7th World Biomaterials Congress. 2004. pp. 1248
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