Development of osteogenic cell sheets for bone tissue engineering applications

Rogério P. Pirraco, Haruko Obokata, Takanori Iwata, Alexandra P. Marques, Satoshi Tsuneda, Masayuki Yamato, Rui L. Reis, Teruo Okano

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    The use of scaffolds in combination with osteogenic cells has been the gold standard in bone tissue engineering strategies. These strategies have, however, in many cases failed to produce the desired results due to issues such as the immunogenicity of the biomaterials used and cell necrosis at the bulk of the scaffold related to deficient oxygen and nutrients diffusion. Here, we originally propose the use of cell sheet (CS) engineering as a possible way to overcome some of these obstacles. Osteogenic CSs were fabricated by culturing rat bone marrow stromal cells in thermoresponsive culture dishes. The CSs were recovered from the dishes using a low-temperature treatment and then were implanted subcutaneously in nude mice. New bone formation was verified from day 7 post-transplantation using X-ray, microcomputed tomography, and histological analysis. The presence of a vascularized marrow was also verified in the newly formed bone after 6 weeks of transplantation. Further, osteocytes were found in this newly formed tissue, supporting the conclusion that mature bone was formed after ectopically transplanting osteogenic CSs. These results therefore confirm the great potentiality of CS engineering to be used in bone tissue engineering applications.

    Original languageEnglish
    Pages (from-to)1507-1515
    Number of pages9
    JournalTissue Engineering - Part A
    Volume17
    Issue number11-12
    DOIs
    Publication statusPublished - 2011 Jun 1

    Fingerprint

    Tissue Engineering
    Tissue engineering
    Bone
    Cell Engineering
    Bone and Bones
    Transplantation
    Scaffolds (biology)
    Osteocytes
    X-Ray Microtomography
    Biocompatible Materials
    Mesenchymal Stromal Cells
    Osteogenesis
    Nude Mice
    Necrosis
    Bone Marrow
    Oxygen
    Biomaterials
    Food
    Nutrients
    Temperature

    ASJC Scopus subject areas

    • Bioengineering
    • Biochemistry
    • Biomedical Engineering
    • Biomaterials
    • Medicine(all)

    Cite this

    Pirraco, R. P., Obokata, H., Iwata, T., Marques, A. P., Tsuneda, S., Yamato, M., ... Okano, T. (2011). Development of osteogenic cell sheets for bone tissue engineering applications. Tissue Engineering - Part A, 17(11-12), 1507-1515. https://doi.org/10.1089/ten.tea.2010.0470

    Development of osteogenic cell sheets for bone tissue engineering applications. / Pirraco, Rogério P.; Obokata, Haruko; Iwata, Takanori; Marques, Alexandra P.; Tsuneda, Satoshi; Yamato, Masayuki; Reis, Rui L.; Okano, Teruo.

    In: Tissue Engineering - Part A, Vol. 17, No. 11-12, 01.06.2011, p. 1507-1515.

    Research output: Contribution to journalArticle

    Pirraco, RP, Obokata, H, Iwata, T, Marques, AP, Tsuneda, S, Yamato, M, Reis, RL & Okano, T 2011, 'Development of osteogenic cell sheets for bone tissue engineering applications', Tissue Engineering - Part A, vol. 17, no. 11-12, pp. 1507-1515. https://doi.org/10.1089/ten.tea.2010.0470
    Pirraco, Rogério P. ; Obokata, Haruko ; Iwata, Takanori ; Marques, Alexandra P. ; Tsuneda, Satoshi ; Yamato, Masayuki ; Reis, Rui L. ; Okano, Teruo. / Development of osteogenic cell sheets for bone tissue engineering applications. In: Tissue Engineering - Part A. 2011 ; Vol. 17, No. 11-12. pp. 1507-1515.
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