Development of the automated circulating tumor cell recovery system with microcavity array

Ryo Negishi, Masahito Hosokawa, Seita Nakamura, Hisashige Kanbara, Masafumi Kanetomo, Yoshihito Kikuhara, Tsuyoshi Tanaka, Tadashi Matsunaga, Tomoko Yoshino

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Circulating tumor cells (CTCs) are well recognized as useful biomarker for cancer diagnosis and potential target of drug discovery for metastatic cancer. Efficient and precise recovery of extremely low concentrations of CTCs from blood has been required to increase the detection sensitivity. Here, an automated system equipped with a microcavity array (MCA) was demonstrated for highly efficient and reproducible CTC recovery. The use of MCA allows selective recovery of cancer cells from whole blood on the basis of differences in size between tumor and blood cells. Intra- and inter-assays revealed that the automated system achieved high efficiency and reproducibility equal to the assay manually performed by well-trained operator. Under optimized assay workflow, the automated system allows efficient and precise cell recovery for non-small cell lung cancer cells spiked in whole blood. The automated CTC recovery system will contribute to high-throughput analysis in the further clinical studies on large cohort of cancer patients.

Original languageEnglish
Pages (from-to)438-442
Number of pages5
JournalBiosensors and Bioelectronics
Volume67
DOIs
Publication statusPublished - 2015 May 5
Externally publishedYes

Keywords

  • Automated system
  • Circulating tumor cells
  • Lung cancer
  • Whole blood

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

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  • Cite this

    Negishi, R., Hosokawa, M., Nakamura, S., Kanbara, H., Kanetomo, M., Kikuhara, Y., Tanaka, T., Matsunaga, T., & Yoshino, T. (2015). Development of the automated circulating tumor cell recovery system with microcavity array. Biosensors and Bioelectronics, 67, 438-442. https://doi.org/10.1016/j.bios.2014.09.002