Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum

Etsuro Ito, Atsuo Miyazawa, Hiroshi Takagi, Tohru Yoshioka, Tetsuro Horikoshi, Keiji Yanagisawa, Takeshi Nakamura, Yoshihisa Kudo, Masato Umeda, Keizo Inoue, Katsuhiko Mikoshiba

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    The postnatal development of calcium-mobilizing systems was studied by both microfluorometric imaging analysis of Ca2+ on living rat cerebellar slices and immunohistochemical labeling of phosphatidylinositol 4,5-bisphosphate (PIP2) and inositol 1,4,5-trisphosphate binding protein (IP3BP) in fixed rat cerebellum. Stimulation with quisqualate (QA) or N-methyl-d-aspartate (NMDA) enhanced the Ca2+ level only diffusely on postnatal day (PND) 3, but more discretely on PNDs 7 and 15. On PND 21, QA-induced responses were localized in the molecular layer especially, but not in the granular layer. By contrast, NMDA mobilized Ca2+ prominently in the granular layer, but only weakly in the molecular layer. Localized expression of PIP2 in the molecular layer paralleled QA-induced Ca2+ mobilization, but IP3BP was expressed more diffusely. The present study offers the first direct evidence that PIP2, but not IP3BP, is essential for QA-induced Ca2+ mobilization in the cerebellar cortex.

    Original languageEnglish
    Pages (from-to)179-188
    Number of pages10
    JournalNeuroscience Research
    Volume11
    Issue number3
    DOIs
    Publication statusPublished - 1991

    Fingerprint

    Quisqualic Acid
    Excitatory Amino Acids
    Cerebellum
    Calcium
    Aspartic Acid
    Cerebellar Cortex
    Inositol 1,4,5-Trisphosphate
    Phosphatidylinositols
    Carrier Proteins

    Keywords

    • Cerebellar slice
    • Development
    • Excitatory amino acid
    • Immunohistochemistry
    • Inositol 1,4,5-trisphosphate binding protein
    • Inositol phospholipid turnover
    • Intracellular calcium concentration
    • Phosphatidylinositol 4,5-bisphosphate

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Ito, E., Miyazawa, A., Takagi, H., Yoshioka, T., Horikoshi, T., Yanagisawa, K., ... Mikoshiba, K. (1991). Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum. Neuroscience Research, 11(3), 179-188. https://doi.org/10.1016/0168-0102(91)90041-V

    Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum. / Ito, Etsuro; Miyazawa, Atsuo; Takagi, Hiroshi; Yoshioka, Tohru; Horikoshi, Tetsuro; Yanagisawa, Keiji; Nakamura, Takeshi; Kudo, Yoshihisa; Umeda, Masato; Inoue, Keizo; Mikoshiba, Katsuhiko.

    In: Neuroscience Research, Vol. 11, No. 3, 1991, p. 179-188.

    Research output: Contribution to journalArticle

    Ito, E, Miyazawa, A, Takagi, H, Yoshioka, T, Horikoshi, T, Yanagisawa, K, Nakamura, T, Kudo, Y, Umeda, M, Inoue, K & Mikoshiba, K 1991, 'Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum', Neuroscience Research, vol. 11, no. 3, pp. 179-188. https://doi.org/10.1016/0168-0102(91)90041-V
    Ito, Etsuro ; Miyazawa, Atsuo ; Takagi, Hiroshi ; Yoshioka, Tohru ; Horikoshi, Tetsuro ; Yanagisawa, Keiji ; Nakamura, Takeshi ; Kudo, Yoshihisa ; Umeda, Masato ; Inoue, Keizo ; Mikoshiba, Katsuhiko. / Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum. In: Neuroscience Research. 1991 ; Vol. 11, No. 3. pp. 179-188.
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