Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum

Etsuro Ito, Atsuo Miyazawa, Hiroshi Takagi, Tohru Yoshioka, Tetsuro Horikoshi, Keiji Yanagisawa, Takeshi Nakamura, Yoshihisa Kudo, Masato Umeda, Keizo Inoue, Katsuhiko Mikoshiba

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14 Citations (Scopus)

Abstract

The postnatal development of calcium-mobilizing systems was studied by both microfluorometric imaging analysis of Ca2+ on living rat cerebellar slices and immunohistochemical labeling of phosphatidylinositol 4,5-bisphosphate (PIP2) and inositol 1,4,5-trisphosphate binding protein (IP3BP) in fixed rat cerebellum. Stimulation with quisqualate (QA) or N-methyl-d-aspartate (NMDA) enhanced the Ca2+ level only diffusely on postnatal day (PND) 3, but more discretely on PNDs 7 and 15. On PND 21, QA-induced responses were localized in the molecular layer especially, but not in the granular layer. By contrast, NMDA mobilized Ca2+ prominently in the granular layer, but only weakly in the molecular layer. Localized expression of PIP2 in the molecular layer paralleled QA-induced Ca2+ mobilization, but IP3BP was expressed more diffusely. The present study offers the first direct evidence that PIP2, but not IP3BP, is essential for QA-induced Ca2+ mobilization in the cerebellar cortex.

Original languageEnglish
Pages (from-to)179-188
Number of pages10
JournalNeuroscience Research
Volume11
Issue number3
DOIs
Publication statusPublished - 1991 Aug

Keywords

  • Cerebellar slice
  • Development
  • Excitatory amino acid
  • Immunohistochemistry
  • Inositol 1,4,5-trisphosphate binding protein
  • Inositol phospholipid turnover
  • Intracellular calcium concentration
  • Phosphatidylinositol 4,5-bisphosphate

ASJC Scopus subject areas

  • Neuroscience(all)

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    Ito, E., Miyazawa, A., Takagi, H., Yoshioka, T., Horikoshi, T., Yanagisawa, K., Nakamura, T., Kudo, Y., Umeda, M., Inoue, K., & Mikoshiba, K. (1991). Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum. Neuroscience Research, 11(3), 179-188. https://doi.org/10.1016/0168-0102(91)90041-V