Developmental changes in ciliary motility on choroid plexus epithelial cells during the perinatal period

Yuta Nonami, Keishi Narita, Hideki Nakamura, Takafumi Inoue, Sen Takeda

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    13 Citations (Scopus)

    Abstract

    Cilia have crucial roles in various developmental and physiological events. Previously, we reported that choroid plexus epithelial cells (CPECs) have multiple, nonmotile 9+0 cilia, but the cilia exhibit transient motility with variable axonemal arrangements in the neonatal period. These features make these cilia unique, as they do not fit in to the traditional categories of primary or motile cilia, and their physiological roles remain elusive. To address this issue, we studied ciliary motility on CPECs through development, with particular interest in the embryonic period. In the fetal choroid plexus of the lateral ventricles, the proportion of cells with motile cilia and their beat frequency increased over time. The ciliary motility profiles peaked near the day of birth, and gradually declined in the two weeks thereafter. The dynamic changes in ciliary motility correlated with changes in Dnahc11 expression. We demonstrated previously that the ciliary motility at P2 was insufficient to produce detectable fluid flow; thus it appears that CPEC cilia do not produce fluid flow at any point during development. Together, our results suggest that a temporally regulated, unique function of CPEC cilia may exist during the perinatal period.

    Original languageEnglish
    Pages (from-to)797-803
    Number of pages7
    JournalCytoskeleton
    Volume70
    Issue number12
    DOIs
    Publication statusPublished - 2013 Dec

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    Keywords

    • Cilia
    • Development
    • Live imaging
    • Real-time polymerase chain reaction (PCR)
    • Scanning electron microscopy

    ASJC Scopus subject areas

    • Cell Biology
    • Structural Biology

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