Developmental changes in progesterone biosynthesis and metabolism in the quail brain

Kazuyoshi Ukena, Yoko Honda, Robert W. Lea, Kazuyoshi Tsutsui

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We have recently demonstrated that the quail brain possesses the cholesterol side-chain cleavage enzyme (cytochrome P450scc) and 3β-hydroxysteroid dehydrogenase/Δ54-isomerase (3β-HSD) and produces pregnenolone, pregnenolone sulfate and progesterone from cholesterol. To elucidate the developmental changes in progesterone biosynthesis and its metabolism in the quail brain, we examined the expression and activity of 3β-HSD and progesterone metabolite(s) during embryonic and post-hatched ages. Both the progesterone concentration and 3β-HSD mRNA expression in the brain were almost constant during embryonic and post-hatched ages. The conversion of pregnenolone to progesterone (net 3β-HSD enzymatic activity) was also constant during development and at maturity. However, without radioinert progesterone, the production of progesterone was drastically reduced in the embryonic brain, indicating active progesterone metabolism at the embryonic stage. Biochemical analysis together with HPLC and TLC revealed that only the embryonic brain actively produced 5β-dihydroprogesterone from progesterone. Thus, progesterone production may be constant during embryonic and post-hatched development and in adulthood, whereas 5β-dihydroprogesterone may be produced actively only in embryonic life due to 5β-reductase.

Original languageEnglish
Pages (from-to)190-194
Number of pages5
JournalBrain Research
Volume898
Issue number1
DOIs
Publication statusPublished - 2001 Apr 13
Externally publishedYes

Fingerprint

Quail
Progesterone
3-Hydroxysteroid Dehydrogenases
Brain
Isomerases
20-alpha-Dihydroprogesterone
Cholesterol Side-Chain Cleavage Enzyme
Pregnenolone
Oxidoreductases
Cholesterol
High Pressure Liquid Chromatography
Messenger RNA

Keywords

  • 3β-Hydroxysteroid dehydrogenase/Δ-δ-isomerase
  • 5β-Dihydroprogesterone
  • 5β-Reductase
  • Development
  • Enzymatic activity
  • Gene expression
  • Progesterone
  • Quail brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Developmental changes in progesterone biosynthesis and metabolism in the quail brain. / Ukena, Kazuyoshi; Honda, Yoko; Lea, Robert W.; Tsutsui, Kazuyoshi.

In: Brain Research, Vol. 898, No. 1, 13.04.2001, p. 190-194.

Research output: Contribution to journalArticle

Ukena, Kazuyoshi ; Honda, Yoko ; Lea, Robert W. ; Tsutsui, Kazuyoshi. / Developmental changes in progesterone biosynthesis and metabolism in the quail brain. In: Brain Research. 2001 ; Vol. 898, No. 1. pp. 190-194.
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