Diallyl disulfide reduced dose-dependently the number of lymphocyte subsets and monocytes in rats

Yoko Hashizume, Ken Shirato, Ikumi Abe, Ayumu Kobayashi, Ryosuke Mitsuhashi, Chikako Shiono, Shogo Sato, Kaoru Tachiyashiki, Kazuhiko Imaizumi

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Diallyl disulfide (DADS) is a major sulfur compound of garlic, and exerts antiinflammatory, immune-modulatory, and enhancing sympathetic activity effects. However, it still remains unclear how DADS affects the distribution of white blood cell subsets, which is essential to execute effective immune responses and partially regulated by adrenal glucocorticoids. Therefore, we examined the dose-dependent effects of DADS administration on the circulating number of white blood cells (WBCs) and lymphocyte subsets, and plasma corticosterone concentration in rats. Male 10-wk-old Sprague Dawley rats were divided into the DADS-free and DADS-orally administered (dose =10, 20, and 40 mg/kg BW) groups. Blood samples were collected from the tail vein at 0, 1, 2, 4, and 6 h after the administration. DADS administration decreased dose- and time-dependently the circulating number of total WBCs, total lympho cytes, and monocytes. Within the lymphocyte subsets, the circulating number of T-lymphocytes and B-lymphocytes was significantly reduced 4 h after DADS administration in a dose-dependent manner, although that of natural killer (NK) cells was not affected. On the other hand, although DADS administration did not significantly change the circulating number of neutrophils, the circulating number of eosinophils and basophils showed a decreasing tendency after DADS administration. In contrast, plasma corticosterone concentration was increased 2 h after DADS administration in a dose-dependent manner. These results suggest that DADS administration reduces the circulating number of monocytes and lymphocytes, including especially acquired immune cells, via the action of corticosterone, and the effects are induced in a dose-dependent manner.

    Original languageEnglish
    Pages (from-to)292-296
    Number of pages5
    JournalJournal of Nutritional Science and Vitaminology
    Volume58
    Issue number4
    Publication statusPublished - 2012

    Fingerprint

    Lymphocyte Subsets
    Monocytes
    Corticosterone
    Leukocytes
    diallyl disulfide
    Sulfur Compounds
    Garlic
    Basophils
    Lymphocyte Count
    Eosinophils
    Natural Killer Cells
    Glucocorticoids
    Sprague Dawley Rats
    Tail
    Veins
    Neutrophils
    B-Lymphocytes
    Anti-Inflammatory Agents
    T-Lymphocytes

    Keywords

    • B-lymphocytes
    • Corticosterone
    • Diallyl disulfide
    • Monocytes
    • T-lymphocytes

    ASJC Scopus subject areas

    • Medicine (miscellaneous)
    • Nutrition and Dietetics

    Cite this

    Hashizume, Y., Shirato, K., Abe, I., Kobayashi, A., Mitsuhashi, R., Shiono, C., ... Imaizumi, K. (2012). Diallyl disulfide reduced dose-dependently the number of lymphocyte subsets and monocytes in rats. Journal of Nutritional Science and Vitaminology, 58(4), 292-296.

    Diallyl disulfide reduced dose-dependently the number of lymphocyte subsets and monocytes in rats. / Hashizume, Yoko; Shirato, Ken; Abe, Ikumi; Kobayashi, Ayumu; Mitsuhashi, Ryosuke; Shiono, Chikako; Sato, Shogo; Tachiyashiki, Kaoru; Imaizumi, Kazuhiko.

    In: Journal of Nutritional Science and Vitaminology, Vol. 58, No. 4, 2012, p. 292-296.

    Research output: Contribution to journalArticle

    Hashizume, Y, Shirato, K, Abe, I, Kobayashi, A, Mitsuhashi, R, Shiono, C, Sato, S, Tachiyashiki, K & Imaizumi, K 2012, 'Diallyl disulfide reduced dose-dependently the number of lymphocyte subsets and monocytes in rats', Journal of Nutritional Science and Vitaminology, vol. 58, no. 4, pp. 292-296.
    Hashizume Y, Shirato K, Abe I, Kobayashi A, Mitsuhashi R, Shiono C et al. Diallyl disulfide reduced dose-dependently the number of lymphocyte subsets and monocytes in rats. Journal of Nutritional Science and Vitaminology. 2012;58(4):292-296.
    Hashizume, Yoko ; Shirato, Ken ; Abe, Ikumi ; Kobayashi, Ayumu ; Mitsuhashi, Ryosuke ; Shiono, Chikako ; Sato, Shogo ; Tachiyashiki, Kaoru ; Imaizumi, Kazuhiko. / Diallyl disulfide reduced dose-dependently the number of lymphocyte subsets and monocytes in rats. In: Journal of Nutritional Science and Vitaminology. 2012 ; Vol. 58, No. 4. pp. 292-296.
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    AU - Shirato, Ken

    AU - Abe, Ikumi

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    AU - Mitsuhashi, Ryosuke

    AU - Shiono, Chikako

    AU - Sato, Shogo

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