Diamond electrolyte solution gate FETs for DNA and protein sensors using DNA/RNA aptamers

Hiroshi Kawarada, A. R. Ruslinda

    Research output: Contribution to journalArticle

    39 Citations (Scopus)

    Abstract

    For DNA and protein detection, we have analyzed DNA/RNA immobilized biosensors based on diamond field effect transistors (FETs), where several types of atomic or molecule termination have been realized on their surfaces. Amine termination and carboxyl termination necessary for biomolecule immobilization can be formed directly on the diamond surfaces resulting in the immobilization of short DNA/RNA within 5nm from the surface. Owing to the high areal capacitance of a liquid electric double layer capacitor and a solid channel capacitor, the change in the surface charge caused by the hybridization of DNA/RNA and the protein binding to DNA/RNA aptamers can be efficiently detected. The charge of surface termination groups such as NH 3 +, O -, COO - affects the suppression of nonspecific binding. The detection of biomolecules such as one-base mismatch of DNA and proteins that is unaffected by nonspecific binding can be realized as a result.

    Original languageEnglish
    Pages (from-to)2005-2016
    Number of pages12
    JournalPhysica Status Solidi (A) Applications and Materials Science
    Volume208
    Issue number9
    DOIs
    Publication statusPublished - 2011 Sep

    Fingerprint

    Nucleotide Aptamers
    Gates (transistor)
    Diamond
    RNA
    Electrolytes
    Diamonds
    DNA
    field effect transistors
    deoxyribonucleic acid
    diamonds
    electrolytes
    proteins
    Proteins
    sensors
    Sensors
    Biomolecules
    immobilization
    Immobilized Nucleic Acids
    RNA-Binding Proteins
    electrochemical capacitors

    Keywords

    • aptamer
    • biosensor
    • diamond
    • DNA
    • FET
    • protein
    • transistor

    ASJC Scopus subject areas

    • Electrical and Electronic Engineering
    • Electronic, Optical and Magnetic Materials
    • Materials Chemistry
    • Condensed Matter Physics
    • Surfaces and Interfaces
    • Surfaces, Coatings and Films

    Cite this

    Diamond electrolyte solution gate FETs for DNA and protein sensors using DNA/RNA aptamers. / Kawarada, Hiroshi; Ruslinda, A. R.

    In: Physica Status Solidi (A) Applications and Materials Science, Vol. 208, No. 9, 09.2011, p. 2005-2016.

    Research output: Contribution to journalArticle

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