Dictyobacter aurantiacus gen. nov., sp. nov., a member of the family Ktedonobacteraceae, isolated from soil, and emended description of the genus thermosporothrix

Shuhei Yabe*, Yasuteru Sakai, Keietsu Abe, Akira Yokota, Akira Take, Atsuko Matsumoto, Arwan Sugiharto, Dwiningsih Susilowati, Moriyuki Hamada, Kazuhide Nara, Made Sudiana, Shigeto Otsuka

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

A mesophilic, Gram-stain-positive, spore-forming bacterium that formed branched mycelia was isolated from paddy soil in Gunung Salak (Mount Salak), West Java, Indonesia. This strain, designated S-27T, grew at temperatures between 20 and 37 °C; the optimum growth temperature was 25 to 30 °C, and no growth was observed at 15 or 45 °C. The pH range for growth was pH 3.5 to 8.6; the optimum pH was 6.0, and no growth was observed at pH 3.0 or 9.2. Strain S-27T was able to hydrolyse polysaccharides such as starch, cellulose and xylan. The G+C content of the DNA of strain S-27T was 55.7 mol%. The major fatty acids were iso-C17: 0 and C16: 1 2-OH, and the major menaquinone was MK-9 (H2). The cell wall of strain S-27T contained Dglutamic acid, glycine, L-alanine, D-alanine, L-ornithine and β-alanine in a molar ratio of 1.0: 1.6: 1.4: 0.6: 0.9: 1.1. The polar lipids consisted of phosphatidylglycerol, phosphatidylinositol and two glycolipids. The major cell-wall sugar was arabinose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S-27T belongs to the order Ktedonobacterales and is most closely related to Ktedonobacter racemifer SOSP1-21T (89.6% sequence identity). On the basis of its chemotaxonomic and phenotypic features and phylogenetic position, we concluded that strain S-27T represents a novel genus and species, for which we propose the name Dictyobacter aurantiacus gen. nov., sp. nov. The type strain of Dictyobacter aurantiacus is strain S-27T (=NBRC 109595T=InaCC B312T). Emendation of the description of the genus Thermosporothrix is also provided.

Original languageEnglish
Article number001985
Pages (from-to)2615-2621
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number8
DOIs
Publication statusPublished - 2017 Jan 1
Externally publishedYes

Keywords

  • Ktedonobacteria

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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