Dictyobacter aurantiacus gen. nov., sp. nov., a member of the family Ktedonobacteraceae, isolated from soil, and emended description of the genus thermosporothrix

Shuhei Yabe, Yasuteru Sakai, Keietsu Abe, Akira Yokota, Akira Take, Atsuko Matsumoto, Arwan Sugiharto, Dwiningsih Susilowati, Moriyuki Hamada, Kazuhide Nara, Made Sudiana, Shigeto Otsuka

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A mesophilic, Gram-stain-positive, spore-forming bacterium that formed branched mycelia was isolated from paddy soil in Gunung Salak (Mount Salak), West Java, Indonesia. This strain, designated S-27T, grew at temperatures between 20 and 37 °C; the optimum growth temperature was 25 to 30 °C, and no growth was observed at 15 or 45 °C. The pH range for growth was pH 3.5 to 8.6; the optimum pH was 6.0, and no growth was observed at pH 3.0 or 9.2. Strain S-27T was able to hydrolyse polysaccharides such as starch, cellulose and xylan. The G+C content of the DNA of strain S-27T was 55.7 mol%. The major fatty acids were iso-C17: 0 and C16: 1 2-OH, and the major menaquinone was MK-9 (H2). The cell wall of strain S-27T contained Dglutamic acid, glycine, L-alanine, D-alanine, L-ornithine and β-alanine in a molar ratio of 1.0: 1.6: 1.4: 0.6: 0.9: 1.1. The polar lipids consisted of phosphatidylglycerol, phosphatidylinositol and two glycolipids. The major cell-wall sugar was arabinose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S-27T belongs to the order Ktedonobacterales and is most closely related to Ktedonobacter racemifer SOSP1-21T (89.6% sequence identity). On the basis of its chemotaxonomic and phenotypic features and phylogenetic position, we concluded that strain S-27T represents a novel genus and species, for which we propose the name Dictyobacter aurantiacus gen. nov., sp. nov. The type strain of Dictyobacter aurantiacus is strain S-27T (=NBRC 109595T=InaCC B312T). Emendation of the description of the genus Thermosporothrix is also provided.

Original languageEnglish
Article number001985
Pages (from-to)2615-2621
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number8
DOIs
Publication statusPublished - 2017 Jan 1
Externally publishedYes

Fingerprint

aprepitant
Soil
new species
Growth
Alanine
Cell Wall
soil
alanylglycine
alanine
phylogenetics
Vitamin K 2
Xylans
Phosphatidylglycerols
Arabinose
Temperature
Ornithine
Indonesia
Mycelium
Glycolipids
Base Composition

Keywords

  • Ktedonobacteria

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Dictyobacter aurantiacus gen. nov., sp. nov., a member of the family Ktedonobacteraceae, isolated from soil, and emended description of the genus thermosporothrix. / Yabe, Shuhei; Sakai, Yasuteru; Abe, Keietsu; Yokota, Akira; Take, Akira; Matsumoto, Atsuko; Sugiharto, Arwan; Susilowati, Dwiningsih; Hamada, Moriyuki; Nara, Kazuhide; Sudiana, Made; Otsuka, Shigeto.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 8, 001985, 01.01.2017, p. 2615-2621.

Research output: Contribution to journalArticle

Yabe, S, Sakai, Y, Abe, K, Yokota, A, Take, A, Matsumoto, A, Sugiharto, A, Susilowati, D, Hamada, M, Nara, K, Sudiana, M & Otsuka, S 2017, 'Dictyobacter aurantiacus gen. nov., sp. nov., a member of the family Ktedonobacteraceae, isolated from soil, and emended description of the genus thermosporothrix', International Journal of Systematic and Evolutionary Microbiology, vol. 67, no. 8, 001985, pp. 2615-2621. https://doi.org/10.1099/ijsem.0.001985
Yabe, Shuhei ; Sakai, Yasuteru ; Abe, Keietsu ; Yokota, Akira ; Take, Akira ; Matsumoto, Atsuko ; Sugiharto, Arwan ; Susilowati, Dwiningsih ; Hamada, Moriyuki ; Nara, Kazuhide ; Sudiana, Made ; Otsuka, Shigeto. / Dictyobacter aurantiacus gen. nov., sp. nov., a member of the family Ktedonobacteraceae, isolated from soil, and emended description of the genus thermosporothrix. In: International Journal of Systematic and Evolutionary Microbiology. 2017 ; Vol. 67, No. 8. pp. 2615-2621.
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