Dielectrophoresis-Assisted SPRF illumination biosensor for selective detection of biological substances

Chiaki Kuroda, Yoshimichi Ohki, Makoto Fujimaki

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    1 Citation (Scopus)

    Abstract

    Aiming at detecting biological substances accurately and speedy, we are developing a dielectrophoresis-Assisted surface plasmon resonance fluorescence (SPRF) illumination biosensor. A sensing chip of the sensor has Al layers, which are used for electrodes and SPR excitation layers. In this study, we used Escherichia coli (E. coli) stained with 4', 6-diamidino-2-phenylindole (DAPI) as a target substance. First, the structure of the sensing chip was optimized to excite SPR on the Al electrodes by 405-nm light. Then, an emulsion with DAPI-stained E. coli was dropped on the sensing chip and illuminated by a 405-nm laser via a prism. When ac voltages were applied to the Al electrodes, the number of bright spots, due to fluorescence from DAPI-stained E. coli, increased on the electrodes. This result indicates that E. coli, which has a lower permittivity than the emulsion, was collected onto the electrodes where the electric field strength is lowest by negative dielectrophoresis. Therefore, we have succeeded in collecting target biological substances on the surface of the sensing chip and detecting them.

    Original languageEnglish
    Title of host publicationCEIDP 2017 - IEEE Conference on Electrical Insulation and Dielectric Phenomenon
    PublisherInstitute of Electrical and Electronics Engineers Inc.
    Pages253-256
    Number of pages4
    Volume2017-October
    ISBN (Electronic)9781538611944
    DOIs
    Publication statusPublished - 2018 Jan 12
    Event2017 IEEE Conference on Electrical Insulation and Dielectric Phenomenon, CEIDP 2017 - Texas, United States
    Duration: 2017 Oct 222017 Oct 25

    Other

    Other2017 IEEE Conference on Electrical Insulation and Dielectric Phenomenon, CEIDP 2017
    CountryUnited States
    CityTexas
    Period17/10/2217/10/25

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Electrical and Electronic Engineering

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  • Cite this

    Kuroda, C., Ohki, Y., & Fujimaki, M. (2018). Dielectrophoresis-Assisted SPRF illumination biosensor for selective detection of biological substances. In CEIDP 2017 - IEEE Conference on Electrical Insulation and Dielectric Phenomenon (Vol. 2017-October, pp. 253-256). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/CEIDP.2017.8257460