Abstract
Aiming at detecting biological substances accurately and speedy, we are developing a dielectrophoresis-Assisted surface plasmon resonance fluorescence (SPRF) illumination biosensor. A sensing chip of the sensor has Al layers, which are used for electrodes and SPR excitation layers. In this study, we used Escherichia coli (E. coli) stained with 4', 6-diamidino-2-phenylindole (DAPI) as a target substance. First, the structure of the sensing chip was optimized to excite SPR on the Al electrodes by 405-nm light. Then, an emulsion with DAPI-stained E. coli was dropped on the sensing chip and illuminated by a 405-nm laser via a prism. When ac voltages were applied to the Al electrodes, the number of bright spots, due to fluorescence from DAPI-stained E. coli, increased on the electrodes. This result indicates that E. coli, which has a lower permittivity than the emulsion, was collected onto the electrodes where the electric field strength is lowest by negative dielectrophoresis. Therefore, we have succeeded in collecting target biological substances on the surface of the sensing chip and detecting them.
| Original language | English |
|---|---|
| Title of host publication | CEIDP 2017 - IEEE Conference on Electrical Insulation and Dielectric Phenomenon |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| Pages | 253-256 |
| Number of pages | 4 |
| Volume | 2017-October |
| ISBN (Electronic) | 9781538611944 |
| DOIs | |
| Publication status | Published - 2018 Jan 12 |
| Event | 2017 IEEE Conference on Electrical Insulation and Dielectric Phenomenon, CEIDP 2017 - Texas, United States Duration: 2017 Oct 22 → 2017 Oct 25 |
Other
| Other | 2017 IEEE Conference on Electrical Insulation and Dielectric Phenomenon, CEIDP 2017 |
|---|---|
| Country | United States |
| City | Texas |
| Period | 17/10/22 → 17/10/25 |
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ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Electrical and Electronic Engineering
Cite this
Dielectrophoresis-Assisted SPRF illumination biosensor for selective detection of biological substances. / Kuroda, Chiaki; Ohki, Yoshimichi; Fujimaki, Makoto.
CEIDP 2017 - IEEE Conference on Electrical Insulation and Dielectric Phenomenon. Vol. 2017-October Institute of Electrical and Electronics Engineers Inc., 2018. p. 253-256.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Dielectrophoresis-Assisted SPRF illumination biosensor for selective detection of biological substances
AU - Kuroda, Chiaki
AU - Ohki, Yoshimichi
AU - Fujimaki, Makoto
PY - 2018/1/12
Y1 - 2018/1/12
N2 - Aiming at detecting biological substances accurately and speedy, we are developing a dielectrophoresis-Assisted surface plasmon resonance fluorescence (SPRF) illumination biosensor. A sensing chip of the sensor has Al layers, which are used for electrodes and SPR excitation layers. In this study, we used Escherichia coli (E. coli) stained with 4', 6-diamidino-2-phenylindole (DAPI) as a target substance. First, the structure of the sensing chip was optimized to excite SPR on the Al electrodes by 405-nm light. Then, an emulsion with DAPI-stained E. coli was dropped on the sensing chip and illuminated by a 405-nm laser via a prism. When ac voltages were applied to the Al electrodes, the number of bright spots, due to fluorescence from DAPI-stained E. coli, increased on the electrodes. This result indicates that E. coli, which has a lower permittivity than the emulsion, was collected onto the electrodes where the electric field strength is lowest by negative dielectrophoresis. Therefore, we have succeeded in collecting target biological substances on the surface of the sensing chip and detecting them.
AB - Aiming at detecting biological substances accurately and speedy, we are developing a dielectrophoresis-Assisted surface plasmon resonance fluorescence (SPRF) illumination biosensor. A sensing chip of the sensor has Al layers, which are used for electrodes and SPR excitation layers. In this study, we used Escherichia coli (E. coli) stained with 4', 6-diamidino-2-phenylindole (DAPI) as a target substance. First, the structure of the sensing chip was optimized to excite SPR on the Al electrodes by 405-nm light. Then, an emulsion with DAPI-stained E. coli was dropped on the sensing chip and illuminated by a 405-nm laser via a prism. When ac voltages were applied to the Al electrodes, the number of bright spots, due to fluorescence from DAPI-stained E. coli, increased on the electrodes. This result indicates that E. coli, which has a lower permittivity than the emulsion, was collected onto the electrodes where the electric field strength is lowest by negative dielectrophoresis. Therefore, we have succeeded in collecting target biological substances on the surface of the sensing chip and detecting them.
UR - http://www.scopus.com/inward/record.url?scp=85045205428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045205428&partnerID=8YFLogxK
U2 - 10.1109/CEIDP.2017.8257460
DO - 10.1109/CEIDP.2017.8257460
M3 - Conference contribution
AN - SCOPUS:85045205428
VL - 2017-October
SP - 253
EP - 256
BT - CEIDP 2017 - IEEE Conference on Electrical Insulation and Dielectric Phenomenon
PB - Institute of Electrical and Electronics Engineers Inc.
ER -