Dietary whey protein hydrolysates increase skeletal muscle glycogen levels via activation of glycogen synthase in mice

Atsushi Kanda, Masashi Morifuji, Tomoyuki Fukasawa, Jinichiro Koga, Minoru Kanegae, Kentaro Kawanaka, Mitsuru Higuchi

Research output: Contribution to journalArticle

7 Citations (Scopus)


Previously, we have shown that consuming carbohydrate plus whey protein hydrolysates (WPHs) replenished muscle glycogen after exercise more effectively than consuming intact whey protein or branched-chain amino acids (BCAAs). The mechanism leading to superior glycogen replenishment after consuming WPH is unclear. In this 5 week intervention, ddY mice were fed experimental diets containing WPH, a mixture of whey amino acids (WAAs), or casein (control). After the intervention, gastrocnemius muscle glycogen levels were significantly higher in the WPH group (4.35 mg/g) than in the WAA (3.15 mg/g) or control (2.51 mg/g) groups. In addition, total glycogen synthase (GS) protein levels were significantly higher in the WPH group (153%) than in the WAA (89.2%) or control groups, and phosphorylated GS levels were significantly decreased in the WPH group (51.4%). These results indicate that dietary WPH may increase the muscle glycogen content through increased GS activity.

Original languageEnglish
Pages (from-to)11403-11408
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Issue number45
Publication statusPublished - 2012 Nov 14



  • exercise
  • glycogen synthase
  • skeletal muscle glycogen
  • Whey protein hydrolysates

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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