Different effects of immobilization stress on the mRNA expression of antioxidant enzymes in rat peripheral organs

Katsutaka Oishi, K. Machida

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    To understand the role of antioxidant enzymatic defenses in reactive oxygen species (ROS) injury following immobilization stress, we examined the effect on mRNA expression of four antioxidant enzymes, manganese superoxide dismutase (Mn-SOD), copper and zinc containing SOD (Cu/Zn-SOD), glutathione peroxidase (GPx) and catalase (CAT), in peripheral rat organs. Immediately after 6 h of immobilization, the mRNA levels of all antioxidants examined were markedly decreased in the liver, while 24 h after the cessation of immobilization, the mRNA expressions recovered to baseline levels. In contrast, the adrenal mRNA levels of Mn-SOD and CAT were increased by stress, and the increased levels of these mRNAs were maintained 24 h after cessation of stress. The stress did not affect the mRNA levels of antioxidants in the heart, lung and kidney. Our results suggest that the antioxidant defense status in peripheral tissues is influenced by immobilization stress and that tissue-specific regulation mechanisms of antioxidant enzymes exist in rats.

    Original languageEnglish
    Pages (from-to)115-121
    Number of pages7
    JournalScandinavian Journal of Clinical and Laboratory Investigation
    Volume62
    Issue number2
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Immobilization
    Rats
    Antioxidants
    Messenger RNA
    Enzymes
    Catalase
    Superoxide Dismutase
    Tissue
    Glutathione Peroxidase
    Liver
    Zinc
    Copper
    Reactive Oxygen Species
    Kidney
    Lung
    Wounds and Injuries

    Keywords

    • Antioxidant enzyme
    • Catalase
    • Glutathione peroxidase
    • Immobilization stress
    • Oxidative stress
    • Rat
    • Superoxide dismutase

    ASJC Scopus subject areas

    • Clinical Biochemistry

    Cite this

    Different effects of immobilization stress on the mRNA expression of antioxidant enzymes in rat peripheral organs. / Oishi, Katsutaka; Machida, K.

    In: Scandinavian Journal of Clinical and Laboratory Investigation, Vol. 62, No. 2, 2002, p. 115-121.

    Research output: Contribution to journalArticle

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