Different target-site specificities of the hairpin ribozyme in cis and trans cleavages

Kazuko Fujitani; Noriko Sasaki-Tozawa; Yo Kikuchi

doi:10.1016/0014-5793(93)80316-M

Different target-site specificities of the hairpin ribozyme in cis and trans cleavages

Kazuko Fujitani, Noriko Sasaki-Tozawa, Yo Kikuchi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The hairpin ribozyme cleaves a phosphodiester bond at the 5' side of a 5'GUC3' sequence of an RNA with high efficiency. An RNA having a 5'GUA3' sequence instead of the GUC sequence is a poor substrate for this ribozyme. Here, we show that this is indeed so in a trans-acting ribozyme system, but in a cis-acting ribozyme system this ribozyme cleaves the 5' side of a GUA sequence as efficiently as the wild-type cleaves the GUC sequence. One base substitution in the ribozyme also affected the target-site specificity in the cis-acting system.

Original language	English
Pages (from-to)	155-158
Number of pages	4
Journal	FEBS Letters
Volume	331
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(93)80316-M
Publication status	Published - 1993 Sept 27
Externally published	Yes

Keywords

Arabis mosaic virus
Catalytic RNA
Chicory yellow mottle virus
Satellite RNA
Tobacco ringspot virus

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0014-5793(93)80316-M

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abstract = "The hairpin ribozyme cleaves a phosphodiester bond at the 5' side of a 5'GUC3' sequence of an RNA with high efficiency. An RNA having a 5'GUA3' sequence instead of the GUC sequence is a poor substrate for this ribozyme. Here, we show that this is indeed so in a trans-acting ribozyme system, but in a cis-acting ribozyme system this ribozyme cleaves the 5' side of a GUA sequence as efficiently as the wild-type cleaves the GUC sequence. One base substitution in the ribozyme also affected the target-site specificity in the cis-acting system.",

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T1 - Different target-site specificities of the hairpin ribozyme in cis and trans cleavages

AU - Fujitani, Kazuko

AU - Sasaki-Tozawa, Noriko

AU - Kikuchi, Yo

PY - 1993/9/27

Y1 - 1993/9/27

N2 - The hairpin ribozyme cleaves a phosphodiester bond at the 5' side of a 5'GUC3' sequence of an RNA with high efficiency. An RNA having a 5'GUA3' sequence instead of the GUC sequence is a poor substrate for this ribozyme. Here, we show that this is indeed so in a trans-acting ribozyme system, but in a cis-acting ribozyme system this ribozyme cleaves the 5' side of a GUA sequence as efficiently as the wild-type cleaves the GUC sequence. One base substitution in the ribozyme also affected the target-site specificity in the cis-acting system.

AB - The hairpin ribozyme cleaves a phosphodiester bond at the 5' side of a 5'GUC3' sequence of an RNA with high efficiency. An RNA having a 5'GUA3' sequence instead of the GUC sequence is a poor substrate for this ribozyme. Here, we show that this is indeed so in a trans-acting ribozyme system, but in a cis-acting ribozyme system this ribozyme cleaves the 5' side of a GUA sequence as efficiently as the wild-type cleaves the GUC sequence. One base substitution in the ribozyme also affected the target-site specificity in the cis-acting system.

KW - Arabis mosaic virus

KW - Catalytic RNA

KW - Chicory yellow mottle virus

KW - Satellite RNA

KW - Tobacco ringspot virus

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Different target-site specificities of the hairpin ribozyme in cis and trans cleavages

Abstract

Keywords

ASJC Scopus subject areas

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