Different target-site specificities of the hairpin ribozyme in cis and trans cleavages

Kazuko Fujitani, Noriko Sasaki-Tozawa, Yo Kikuchi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The hairpin ribozyme cleaves a phosphodiester bond at the 5' side of a 5'GUC3' sequence of an RNA with high efficiency. An RNA having a 5'GUA3' sequence instead of the GUC sequence is a poor substrate for this ribozyme. Here, we show that this is indeed so in a trans-acting ribozyme system, but in a cis-acting ribozyme system this ribozyme cleaves the 5' side of a GUA sequence as efficiently as the wild-type cleaves the GUC sequence. One base substitution in the ribozyme also affected the target-site specificity in the cis-acting system.

Original languageEnglish
Pages (from-to)155-158
Number of pages4
JournalFEBS Letters
Volume331
Issue number1-2
DOIs
Publication statusPublished - 1993 Sep 27
Externally publishedYes

Fingerprint

Catalytic RNA
RNA
Substitution reactions
hairpin ribozyme
Substrates

Keywords

  • Arabis mosaic virus
  • Catalytic RNA
  • Chicory yellow mottle virus
  • Satellite RNA
  • Tobacco ringspot virus

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Different target-site specificities of the hairpin ribozyme in cis and trans cleavages. / Fujitani, Kazuko; Sasaki-Tozawa, Noriko; Kikuchi, Yo.

In: FEBS Letters, Vol. 331, No. 1-2, 27.09.1993, p. 155-158.

Research output: Contribution to journalArticle

Fujitani, Kazuko ; Sasaki-Tozawa, Noriko ; Kikuchi, Yo. / Different target-site specificities of the hairpin ribozyme in cis and trans cleavages. In: FEBS Letters. 1993 ; Vol. 331, No. 1-2. pp. 155-158.
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