Abstract
We have developed an on-chip microcultivation system for differential analysis of photosynthesizing cells of Chlamydomonas as a means of comparing the difference in interdivision time between sister cells and cells of adjacent generations having the same DNA and environment. The system enabled us to compare the change in the interdivision time of four sister cells simultaneously for several days, we found that the interdivision times of the four sister cells synchronize well, while, those of cells of subsequent generations do not. The result showed the potential of the system for measuring how much epigenetic information of eukaryotic cells can be conserved between sister cells and cells of subsequent generations.
| Original language | English |
|---|---|
| Pages (from-to) | L784-L787 |
| Journal | Japanese Journal of Applied Physics, Part 2: Letters |
| Volume | 42 |
| Issue number | 7 A |
| DOIs | |
| Publication status | Published - 2003 Jul 1 |
| Externally published | Yes |
Keywords
- Bacteria
- Cell cycle
- Chlamydomonas
- Differential analysis
- Genome
- Microchamber
- Microcultivation
- Single cell
ASJC Scopus subject areas
- Engineering(all)
- Physics and Astronomy (miscellaneous)
- Physics and Astronomy(all)
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Cite this
Matsumura, K., Yagi, T., & Yasuda, K. (2003). Differential analysis of cell cycle stability in Chlamydomonas using on-chip single-cell cultivation system. Japanese Journal of Applied Physics, Part 2: Letters, 42(7 A), L784-L787. https://doi.org/10.1143/jjap.42.l784