Differential analysis of cell cycle stability in Chlamydomonas using on-chip single-cell cultivation system

Kazunori Matsumura; Toshiki Yagi; Kenji Yasuda

doi:10.1143/jjap.42.l784

Differential analysis of cell cycle stability in Chlamydomonas using on-chip single-cell cultivation system

Kazunori Matsumura, Toshiki Yagi, Kenji Yasuda

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

We have developed an on-chip microcultivation system for differential analysis of photosynthesizing cells of Chlamydomonas as a means of comparing the difference in interdivision time between sister cells and cells of adjacent generations having the same DNA and environment. The system enabled us to compare the change in the interdivision time of four sister cells simultaneously for several days, we found that the interdivision times of the four sister cells synchronize well, while, those of cells of subsequent generations do not. The result showed the potential of the system for measuring how much epigenetic information of eukaryotic cells can be conserved between sister cells and cells of subsequent generations.

Original language	English
Pages (from-to)	L784-L787
Journal	Japanese Journal of Applied Physics, Part 2: Letters
Volume	42
Issue number	7 A
DOIs	https://doi.org/10.1143/jjap.42.l784
Publication status	Published - 2003 Jul 1
Externally published	Yes

Keywords

Bacteria
Cell cycle
Chlamydomonas
Differential analysis
Genome
Microchamber
Microcultivation
Single cell

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy (miscellaneous)
Physics and Astronomy(all)

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10.1143/jjap.42.l784

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abstract = "We have developed an on-chip microcultivation system for differential analysis of photosynthesizing cells of Chlamydomonas as a means of comparing the difference in interdivision time between sister cells and cells of adjacent generations having the same DNA and environment. The system enabled us to compare the change in the interdivision time of four sister cells simultaneously for several days, we found that the interdivision times of the four sister cells synchronize well, while, those of cells of subsequent generations do not. The result showed the potential of the system for measuring how much epigenetic information of eukaryotic cells can be conserved between sister cells and cells of subsequent generations.",

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AU - Yagi, Toshiki

AU - Yasuda, Kenji

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AB - We have developed an on-chip microcultivation system for differential analysis of photosynthesizing cells of Chlamydomonas as a means of comparing the difference in interdivision time between sister cells and cells of adjacent generations having the same DNA and environment. The system enabled us to compare the change in the interdivision time of four sister cells simultaneously for several days, we found that the interdivision times of the four sister cells synchronize well, while, those of cells of subsequent generations do not. The result showed the potential of the system for measuring how much epigenetic information of eukaryotic cells can be conserved between sister cells and cells of subsequent generations.

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KW - Cell cycle

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KW - Genome

KW - Microchamber

KW - Microcultivation

KW - Single cell

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