Differential daily expression of Per1 and Per2 mRNA in the suprachiasmatic nucleus of fetal and early postnatal mice

Haruka Shimomura, Takahiro Moriya, Motoki Sudo, Hisanori Wakamatsu, Masashi Akiyama, Yoshiaki Miyake, Shigenobu Shibata

    Research output: Contribution to journalArticle

    58 Citations (Scopus)

    Abstract

    It is well known that there are circadian rhythms of 2-deoxyglucose uptake and neuronal firing in the rat suprachiasmatic nucleus (SCN) during fetal and early postnatal periods. A core clock mechanism in the mouse SCN appears to involve a transcriptional feedback loop in which CLOCK and BMAL1 function as positive regulators and three mPeriod (mPer) genes play a role in negative feedback. Per genes expression occurs not only in the adult SCN but also in the fetal SCN. However, the developmental change in these genes remains unclear. In this experiment, we examined the day-night pattern of expression of Per1 and Per2 mRNA in the mouse SCN and cerebral cortex on embryonic day 17, postnatal day 3, and in young adult mice under a light-dark cycle. Daily rhythms of mRNA content were observed in mPer1 but not mPer2 in the fetal SCN. Interestingly, the expression of mPer2 in the SCN was high throughout the entire day, and a significant daily rhythm of this gene was observed on postnatal day 6. The expression pattern of SCN mPer1 in constant darkness was similar to that seen in the light-dark cycle. The present results suggest that the daily oscillation of mPer1 but not of mPer2 in the SCN in fetal and early postnatal mice may be associated with the daily rhythms of 2-deoxyglucose uptake and neuronal firing.

    Original languageEnglish
    Pages (from-to)687-693
    Number of pages7
    JournalEuropean Journal of Neuroscience
    Volume13
    Issue number4
    DOIs
    Publication statusPublished - 2001

    Fingerprint

    Suprachiasmatic Nucleus
    Messenger RNA
    Deoxyglucose
    Photoperiod
    Genes
    Darkness
    Circadian Rhythm
    Cerebral Cortex
    Young Adult
    Gene Expression

    Keywords

    • Cerebral cortex
    • Circadian rhythm
    • Development
    • Fetal
    • Per
    • Suprachiasmatic nucleus

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Differential daily expression of Per1 and Per2 mRNA in the suprachiasmatic nucleus of fetal and early postnatal mice. / Shimomura, Haruka; Moriya, Takahiro; Sudo, Motoki; Wakamatsu, Hisanori; Akiyama, Masashi; Miyake, Yoshiaki; Shibata, Shigenobu.

    In: European Journal of Neuroscience, Vol. 13, No. 4, 2001, p. 687-693.

    Research output: Contribution to journalArticle

    Shimomura, Haruka ; Moriya, Takahiro ; Sudo, Motoki ; Wakamatsu, Hisanori ; Akiyama, Masashi ; Miyake, Yoshiaki ; Shibata, Shigenobu. / Differential daily expression of Per1 and Per2 mRNA in the suprachiasmatic nucleus of fetal and early postnatal mice. In: European Journal of Neuroscience. 2001 ; Vol. 13, No. 4. pp. 687-693.
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