Differential display analysis of gene expression in female-to-male sex-reversing gonads of the frog Rana rugosa

Goro Okada, Koichi Maruo, Sadahiro Funada, Masahisa Nakamura

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Sex steroids play pivotal roles in gonadal differentiation in many species of vertebrates. The sex can be reversed from female to male by testosterone in the Japanese wrinkled frog Rana rugosa, but it is still unclear what genes are up- or down-regulated during the XX sex-reversal in this species. To search the genes for the female-to-male sex-reversal, we employed differential display and 5′/3′-RACE. Consequently, we isolated from the gonads at day 8 after testosterone injection 24 different cDNA fragments showing a testosterone treatment-related change and then obtained three full-length cDNAs, which we termed Zfp64, Zfp112, and Rrp54. The former two cDNAs encoded different proteins with zinc-finger domains, whereas the latter cDNA encoded an unknown protein. Transcripts of the three genes were hardly detectable in the sex-reversing gonads at day 24 after the injection; at this time few growing oocytes were observed in the sex-reversing gonad. Besides, in situ hybridization analysis showed positive signals of the three genes in the cytoplasm of growing oocytes of an ovary when testosterone was injected into a tadpole. Thus, the decrease in expression of these three genes was probably due to the disappearance of growing oocytes and not to their direct involvement in the testis formation. To find the key-gene for testis formation, it will be necessary to analyze, by the differential display method, more genes showing a change in expression pattern during sex reversal.

    Original languageEnglish
    Pages (from-to)623-634
    Number of pages12
    JournalGeneral and Comparative Endocrinology
    Volume155
    Issue number3
    DOIs
    Publication statusPublished - 2008 Feb 1

    Fingerprint

    Ranidae
    Gonads
    Anura
    gonads
    frogs
    Gene Expression
    gene expression
    Testosterone
    gender
    testosterone
    Complementary DNA
    sex reversal
    Genes
    Oocytes
    genes
    oocytes
    Testis
    46, XX Testicular Disorders of Sex Development
    testes
    Injections

    Keywords

    • Amphibians
    • Differential display
    • In situ hybridization
    • Oocytes
    • Rana rugosa
    • Rrp54
    • Zfp112
    • Zfp64

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Differential display analysis of gene expression in female-to-male sex-reversing gonads of the frog Rana rugosa. / Okada, Goro; Maruo, Koichi; Funada, Sadahiro; Nakamura, Masahisa.

    In: General and Comparative Endocrinology, Vol. 155, No. 3, 01.02.2008, p. 623-634.

    Research output: Contribution to journalArticle

    Okada, Goro ; Maruo, Koichi ; Funada, Sadahiro ; Nakamura, Masahisa. / Differential display analysis of gene expression in female-to-male sex-reversing gonads of the frog Rana rugosa. In: General and Comparative Endocrinology. 2008 ; Vol. 155, No. 3. pp. 623-634.
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