Differential effects of progesterone and 17β-estradiol on the Ca2+ entry induced by thapsigargin and endothelin-1 in in situ endothelial cells

Junko Y. Toshima, Katsuya Hirano, Junji Nishimura, Hitoo Nakano, Hideo Kanaide

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    The effects of progesterone and 17β-estradiol on Ca2+ signaling in in situ endothelial cells were investigated using front-surface fluorometry of fura-2-loaded strips of porcine aortic valve. Progesterone inhibited the thapsigargin-induced sustained [Ca2+](i) elevation (IC50 = 33.9 μM, n = 4), while 17β-estradiol added a transient [Ca2+](i) elevation. Progesterone and 17β-estradiol had no significant effect on the thapsigargin-induced [Ca2+](i) elevations in the absence of extracellular Ca2+. A Mn2+-induced decline of fluorescent intensity at 360 nm excitation was accelerated by thapsigargin. This acceleration was completely reversed by progesterone, but not by 17β-estradiol. Progesterone inhibited, and 17β-estradiol enhanced the endothelin-1 (ET-1)-induced [Ca2+](i) elevation, while both had no effect on the ET-1-induced Ca2+ release observed in the absence of extracellular Ca2+ or in the pertussis toxin-treated strips. Progesterone and 17β-estradiol thus had different effects on Ca2+ signaling, especially on Ca2+ influx, in endothelial cells. (C) 2000 Elsevier Science B.V.

    Original languageEnglish
    Pages (from-to)109-121
    Number of pages13
    JournalBiochimica et Biophysica Acta - Molecular Cell Research
    Volume1499
    Issue number1-2
    DOIs
    Publication statusPublished - 2000 Dec 11

    Fingerprint

    Thapsigargin
    Endothelin-1
    Progesterone
    Estradiol
    Endothelial Cells
    Fluorometry
    Fura-2
    Pertussis Toxin
    Aortic Valve
    Inhibitory Concentration 50
    Swine

    Keywords

    • 17β-Estradiol
    • Calcium influx
    • Endothelial cell
    • Manganese quenching
    • Progesterone
    • Thapsigargin

    ASJC Scopus subject areas

    • Cell Biology
    • Molecular Biology
    • Biophysics

    Cite this

    Differential effects of progesterone and 17β-estradiol on the Ca2+ entry induced by thapsigargin and endothelin-1 in in situ endothelial cells. / Toshima, Junko Y.; Hirano, Katsuya; Nishimura, Junji; Nakano, Hitoo; Kanaide, Hideo.

    In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1499, No. 1-2, 11.12.2000, p. 109-121.

    Research output: Contribution to journalArticle

    Toshima, Junko Y. ; Hirano, Katsuya ; Nishimura, Junji ; Nakano, Hitoo ; Kanaide, Hideo. / Differential effects of progesterone and 17β-estradiol on the Ca2+ entry induced by thapsigargin and endothelin-1 in in situ endothelial cells. In: Biochimica et Biophysica Acta - Molecular Cell Research. 2000 ; Vol. 1499, No. 1-2. pp. 109-121.
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