Differential expression of poly(ADP-ribose) polymerase and DNA polymerase β in rat tissues

Marta Menegazzi, Gigliola Grassi-Zucconi, Alessandra Carcereri De Prati, Tsutomu Ogura, Palmiro Poltronieri, Hiroshi Nyunoya, Yuko Shiratori-Nyunoya, Masanao Miwa, Hisanori Suzuki

Research output: Contribution to journalArticle

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Abstract

The activities of two DNA repair-related enzymes, poly(ADP-ribose) polymerase and DNA polymerase β, and their mRNA levels were measured in 17 tissues of Wistar rats. A large variety in enzyme activity values could be detected in the tissues examined; the highest levels of activity for both enzymes were found in the testis. A good correlation between poly(ADP-ribose) polymerase activity and the level of the transcript of the gene coding for the enzyme was observed in many tissues. A less satisfactory correlation could be evidenced for DNA polymerase β. The almost parallel amounts of the mRNAs for poly(ADP-ribose) polymerase and DNA polymerase β in the tissues examined suggest a possible coexpression of the genes coding for these enzymes. Additional studies have been carried out in testis and liver by immunohistochemical techniques and byin situ hybridization analyses. While in the testis the spermatocytes were shown to contain both enzymes and their transcripts, in other types of cells this could not be observed. In the liver mRNAs and enzymes were only found in 20% of the hepatocytes. This may in part explain both the low levels of the mRNAs and the modest activities of the two enzymes in that tissue.

Original languageEnglish
Pages (from-to)66-74
Number of pages9
JournalExperimental Cell Research
Volume197
Issue number1
DOIs
Publication statusPublished - 1991
Externally publishedYes

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Poly(ADP-ribose) Polymerases
DNA-Directed DNA Polymerase
Enzymes
Testis
Messenger RNA
DNA Repair Enzymes
Spermatocytes
Liver
Genes
Wistar Rats
Hepatocytes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Menegazzi, M., Grassi-Zucconi, G., De Prati, A. C., Ogura, T., Poltronieri, P., Nyunoya, H., ... Suzuki, H. (1991). Differential expression of poly(ADP-ribose) polymerase and DNA polymerase β in rat tissues. Experimental Cell Research, 197(1), 66-74. https://doi.org/10.1016/0014-4827(91)90480-I

Differential expression of poly(ADP-ribose) polymerase and DNA polymerase β in rat tissues. / Menegazzi, Marta; Grassi-Zucconi, Gigliola; De Prati, Alessandra Carcereri; Ogura, Tsutomu; Poltronieri, Palmiro; Nyunoya, Hiroshi; Shiratori-Nyunoya, Yuko; Miwa, Masanao; Suzuki, Hisanori.

In: Experimental Cell Research, Vol. 197, No. 1, 1991, p. 66-74.

Research output: Contribution to journalArticle

Menegazzi, M, Grassi-Zucconi, G, De Prati, AC, Ogura, T, Poltronieri, P, Nyunoya, H, Shiratori-Nyunoya, Y, Miwa, M & Suzuki, H 1991, 'Differential expression of poly(ADP-ribose) polymerase and DNA polymerase β in rat tissues', Experimental Cell Research, vol. 197, no. 1, pp. 66-74. https://doi.org/10.1016/0014-4827(91)90480-I
Menegazzi, Marta ; Grassi-Zucconi, Gigliola ; De Prati, Alessandra Carcereri ; Ogura, Tsutomu ; Poltronieri, Palmiro ; Nyunoya, Hiroshi ; Shiratori-Nyunoya, Yuko ; Miwa, Masanao ; Suzuki, Hisanori. / Differential expression of poly(ADP-ribose) polymerase and DNA polymerase β in rat tissues. In: Experimental Cell Research. 1991 ; Vol. 197, No. 1. pp. 66-74.
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