Abstract
We studied the regulation of the β-galactoside α2,6-sialyltransferase (hST6Gal I) gene during HL-60 cell differentiation induced with dimethyl sulfoxide (DMSO), all trans-retinoic acid (ATRA), and phorbol myristate acetate (PMA). During HL-60 cell line differentiation, cell surface levels of α2,6-sialic acids expression decreased, as measured by flow cytometric analysis using Sambucus nigra agglutinin (SNA). Activities of hST6Gal I and levels of hST6Gal I mRNA dramatically decreased after 1 day of stimulation. Using reverse transcription polymerase chain reaction (PT-PCR), we found the major hST6Gal I mRNA isoform in HL-60 cells contains 5'-untranslated exons Y and Z. These results suggest that the expression of cell surface α2,6-sialic acids is controlled at the mRNA level, which is regulated by a promoter located 5'-upstream of exon Y. Copyright (C) 1998 Federation of European Biochemical Societies.
| Original language | English |
|---|---|
| Pages (from-to) | 191-194 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 441 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1998 Dec 18 |
| Externally published | Yes |
Keywords
- Alternative splicing
- Cell differentiation
- Gene expression
- HL-60 cell
- Sambucus nigra agglutinin
- Sialyltransferase
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology