Differentiation elicits negative regulation of human β-galactoside α2,6-sialyltransferase at the mRNA level in the HL-60 cell line

Akiyoshi Taniguchi, Koji Higai, Yuko Hasegawa, Kunio Utsumi, Kojiro Matsumoto

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Abstract

We studied the regulation of the β-galactoside α2,6-sialyltransferase (hST6Gal I) gene during HL-60 cell differentiation induced with dimethyl sulfoxide (DMSO), all trans-retinoic acid (ATRA), and phorbol myristate acetate (PMA). During HL-60 cell line differentiation, cell surface levels of α2,6-sialic acids expression decreased, as measured by flow cytometric analysis using Sambucus nigra agglutinin (SNA). Activities of hST6Gal I and levels of hST6Gal I mRNA dramatically decreased after 1 day of stimulation. Using reverse transcription polymerase chain reaction (PT-PCR), we found the major hST6Gal I mRNA isoform in HL-60 cells contains 5'-untranslated exons Y and Z. These results suggest that the expression of cell surface α2,6-sialic acids is controlled at the mRNA level, which is regulated by a promoter located 5'-upstream of exon Y. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)191-194
Number of pages4
JournalFEBS Letters
Volume441
Issue number2
DOIs
Publication statusPublished - 1998 Dec 18

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Keywords

  • Alternative splicing
  • Cell differentiation
  • Gene expression
  • HL-60 cell
  • Sambucus nigra agglutinin
  • Sialyltransferase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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