Digital fabrication of 3D bio devices utilizing PELID (Patterning with Electrostatically-Injected Droplet) method

Shinjiro Umezu, Tatsuru Hatta, Hitoshi Ohmori

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we fabricated soft 3D bio devices utilizing PELID (Patterning with Electrostatically-Injected Droplet) method. It is preferable to perform laboratory experiments with 3D structures in bioengineering. We have investigated mechanism and fundamental characteristics of the PELID method and now been applying for new printing technology of high image quality and 3D printing technology. The method has two merits, higher resolution than commercial printer and ability to eject with highly viscous liquid. We can eject viscous paste that viscosity is 30000 mPas. At DF 2010, I already presented that cells and scaffolds were printed to fabricate 3D cell structures because scaffolds assisted the weight of cells. Now, we should fabricate 3D structure that has cave because real 3D structure has blood vessel like cave. It is difficult to fabricate 3D structure that has cave. Gelatin is used as sacrificial layer. When the printed 3D structure is put into hot water, gelatin is removed. With this technique, we can print 3D structure that has cave. The tube filled with the liquid that contained gelatin and the tube filled with the liquid that contained calcium alginate was hanged down perpendicular to a dish. Voltage was applied between the syringes and the dish by power supplies (voltage range: -5kV ∼ +5kV, Matsusada Precision Inc, Tokyo, HVR-10P). The air gap was adjusted by a z-stage and the plate electrode was moved in x and y directions with two linear motors. PC controlled voltage application and motion of linear stages. We fabricated 3D bio devices.

Original languageEnglish
Title of host publicationNIP 2012
Subtitle of host publication28th International Conference on Digital Printing Technologies and Digital Fabrication 2012 - Technical Program and Proceedings
Pages338-340
Number of pages3
Publication statusPublished - 2012 Dec 1
Externally publishedYes
Event28th International Conference on Digital Printing Technologies, NIP 2012 and Digital Fabrication 2012 - Quebec City, QC, Canada
Duration: 2012 Sep 92012 Sep 13

Publication series

NameInternational Conference on Digital Printing Technologies

Conference

Conference28th International Conference on Digital Printing Technologies, NIP 2012 and Digital Fabrication 2012
CountryCanada
CityQuebec City, QC
Period12/9/912/9/13

ASJC Scopus subject areas

  • Media Technology
  • Computer Science Applications

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