Dipeptide synthesis by l-amino acid ligase from Ralstonia solanacearum

Kuniki Kino, Yuji Nakazawa, Makoto Yagasaki

    Research output: Contribution to journalArticle

    28 Citations (Scopus)

    Abstract

    Despite its utility, dipeptides have not been widely used due to the absence of an efficient manufacturing method. Recently, a novel method for effective production of dipeptides using l-amino acid α-ligase (Lal) is presented. Lal, which is only identified in Bacillus subtilis, catalyzes dipeptide synthesis from unprotected amino acids in an ATP-dependent manner. However, not all the dipeptide can be synthesized by Lal from B. subtilis (BsLal) due to its substrate specificity. Here, we attempted to find a novel Lal exhibiting different substrate specificity from BsLal. By in silico screening based on the amino acid sequence of BsLal, RSp1486a an unknown protein from Ralstonia solanacearum was found to show the Lal activity. RSp1486a exhibited different substrate specificity from BsLal, and preferably synthesized hetero-dipeptides where more bulky amino acid was placed at N terminus and less bulky amino acid was placed at C terminus in opposition to those synthesized by BsLal.

    Original languageEnglish
    Pages (from-to)536-540
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Volume371
    Issue number3
    DOIs
    Publication statusPublished - 2008 Jul 4

    Fingerprint

    Ralstonia solanacearum
    Dipeptides
    Ligases
    Amino Acids
    Substrate Specificity
    Bacillus subtilis
    Substrates
    Computer Simulation
    Bacilli
    Amino Acid Sequence
    Adenosine Triphosphate
    Screening

    Keywords

    • Dipeptide
    • l-Amino acid α-ligase
    • Ralstonia solanacearum

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Dipeptide synthesis by l-amino acid ligase from Ralstonia solanacearum. / Kino, Kuniki; Nakazawa, Yuji; Yagasaki, Makoto.

    In: Biochemical and Biophysical Research Communications, Vol. 371, No. 3, 04.07.2008, p. 536-540.

    Research output: Contribution to journalArticle

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