Direct Count of Bacteria Using Fluorescent Dyes

Application to Assessment of Electrochemical Disinfection

Tadashi Matsunaga, Mina Okochi, Satoshi Nakasono

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A rapid method for direct counting of viable and dead cells using two fluorescent dyes, propidium iodide and 4 ', 6-diamidino-2-phenylindole, was developed for estimation of cell viability after electrochemical disinfection. Using this double staining method, dead and viable cells were visualized as red and blue fluorescent cells, respectively, under UV excitation employing epifluorescence microscopy. A good correlation between the red cell ratio and the ratio of dead cells determined by the plate count method was obtained for Vibrio alginolyticus cells exposed to heat (60 °C), 10 ppm of chlorine, 3.7% of formaldehyde, and electrochemical disinfection treatment.

Original languageEnglish
Pages (from-to)4487-4490
Number of pages4
JournalAnalytical Chemistry
Volume67
Issue number24
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Disinfection
Fluorescent Dyes
Bacteria
Cells
Propidium
Chlorine
Formaldehyde
Microscopic examination
Hot Temperature
DAPI

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Direct Count of Bacteria Using Fluorescent Dyes : Application to Assessment of Electrochemical Disinfection. / Matsunaga, Tadashi; Okochi, Mina; Nakasono, Satoshi.

In: Analytical Chemistry, Vol. 67, No. 24, 1995, p. 4487-4490.

Research output: Contribution to journalArticle

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