Direct formation of homogeneous DNA-probe surface on polyimide thin film by vapor deposition polymerization

Atsushi Kira, Hyonchol Kim, Yoshio Hasegawa, Yoshikazu Takahashi, Kazunori Okano, Kenji Yasuda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

For the single-molecule level quantitative measurement of target mRNAs attached on the DNA chip, one of the most important things is how to maintain the homogeneous, desirable-spaced arrangement of DNA-probes on the surface of the chip. Thus we have developed the novel method for decorating DNA-probes on the chip with desired intervals and homogeneous concentration applying the vapor deposition polymerization (VDP) technique, in which hexamethylene diisocyanate (HDI) and 3,5-diaminobenzonic acid (DBA) molecules were polymerized directly on the chip to form the polymer thin layer. The interval of the reactive carboxyl-group (-COOH) on the side-chains of DBA was controlled to become 2.0 nm by choosing another molecule having this length (HDI). After we made 10-nm-thick HDI-DBA polymer film on the chip, we attached amine-group of the thiol-, amine-decorated DNA-probe to the carboxyl-group of DBA. Then we checked the spatial arrangement and homogeneity of DNA-probes attached on the polymer film using 20-nm gold (Au) nano-particles. By the scanning electric microscopy observation, we first confirmed the spatial distribution of Au nano-particles on the chip was 254 particles/μ m2, whereas the less than 1 particles/μ m2 for the control sample, in which no DNA-probe was attached on the film. Moreover, the standard deviation of localization of particle distribution was less than 8 particles differences / 254 particles/μ m2, which is a magnitude better than the conventional silane-coupling method. The result indicates the potential of our method to form the homogeneous DNA-probe surface on the.

Original languageEnglish
Title of host publicationDigest of Papers - Microprocesses and Nanotechnology 2005
Subtitle of host publication2005 International Microprocesses and Nanotechnology Conference
Number of pages1
Publication statusPublished - 2005 Dec 1
Event2005 International Microprocesses and Nanotechnology Conference - Tokyo, Japan
Duration: 2005 Oct 252005 Oct 28

Publication series

NameDigest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference
Volume2005

Conference

Conference2005 International Microprocesses and Nanotechnology Conference
CountryJapan
CityTokyo
Period05/10/2505/10/28

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ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kira, A., Kim, H., Hasegawa, Y., Takahashi, Y., Okano, K., & Yasuda, K. (2005). Direct formation of homogeneous DNA-probe surface on polyimide thin film by vapor deposition polymerization. In Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference [1595285] (Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference; Vol. 2005).