Direct immobilization of DNA on partially functionalized diamond surface

Jung Hoon Yang, Kwang Soup Song, Shouma Kuga, Hiroshi Kawarada

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    1 Citation (Scopus)

    Abstract

    Amino groups were functionalized directly on the diamond surface after treating oxidation and fluorination for detection of DNAs, respectively. For simple process, immobilization of probe DNAs was carried out directly on the partially aminated diamond without linker molecules. After fabricating micropattemed diamond, specific hybridization with Cy-5 labeled target DNA at a concentration of 100 nM could be clearly detected on H-terminated, partially O-terminated, and partially F-terminated diamonds, respectively. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.

    Original languageEnglish
    Title of host publicationMaterials Research Society Symposium Proceedings
    Pages220-227
    Number of pages8
    Volume950
    Publication statusPublished - 2006
    Event2006 MRS Fall Meeting - Boston, MA
    Duration: 2006 Nov 272006 Dec 1

    Other

    Other2006 MRS Fall Meeting
    CityBoston, MA
    Period06/11/2706/12/1

    Fingerprint

    Diamond
    Diamonds
    DNA
    Fluorination
    DNA Probes
    Microscopic examination
    Oxidation
    Molecules

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials

    Cite this

    Yang, J. H., Song, K. S., Kuga, S., & Kawarada, H. (2006). Direct immobilization of DNA on partially functionalized diamond surface. In Materials Research Society Symposium Proceedings (Vol. 950, pp. 220-227)

    Direct immobilization of DNA on partially functionalized diamond surface. / Yang, Jung Hoon; Song, Kwang Soup; Kuga, Shouma; Kawarada, Hiroshi.

    Materials Research Society Symposium Proceedings. Vol. 950 2006. p. 220-227.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Yang, JH, Song, KS, Kuga, S & Kawarada, H 2006, Direct immobilization of DNA on partially functionalized diamond surface. in Materials Research Society Symposium Proceedings. vol. 950, pp. 220-227, 2006 MRS Fall Meeting, Boston, MA, 06/11/27.
    Yang JH, Song KS, Kuga S, Kawarada H. Direct immobilization of DNA on partially functionalized diamond surface. In Materials Research Society Symposium Proceedings. Vol. 950. 2006. p. 220-227
    Yang, Jung Hoon ; Song, Kwang Soup ; Kuga, Shouma ; Kawarada, Hiroshi. / Direct immobilization of DNA on partially functionalized diamond surface. Materials Research Society Symposium Proceedings. Vol. 950 2006. pp. 220-227
    @inproceedings{3143ede9d9834012a27cb1fdc914e17d,
    title = "Direct immobilization of DNA on partially functionalized diamond surface",
    abstract = "Amino groups were functionalized directly on the diamond surface after treating oxidation and fluorination for detection of DNAs, respectively. For simple process, immobilization of probe DNAs was carried out directly on the partially aminated diamond without linker molecules. After fabricating micropattemed diamond, specific hybridization with Cy-5 labeled target DNA at a concentration of 100 nM could be clearly detected on H-terminated, partially O-terminated, and partially F-terminated diamonds, respectively. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.",
    author = "Yang, {Jung Hoon} and Song, {Kwang Soup} and Shouma Kuga and Hiroshi Kawarada",
    year = "2006",
    language = "English",
    isbn = "9781604234060",
    volume = "950",
    pages = "220--227",
    booktitle = "Materials Research Society Symposium Proceedings",

    }

    TY - GEN

    T1 - Direct immobilization of DNA on partially functionalized diamond surface

    AU - Yang, Jung Hoon

    AU - Song, Kwang Soup

    AU - Kuga, Shouma

    AU - Kawarada, Hiroshi

    PY - 2006

    Y1 - 2006

    N2 - Amino groups were functionalized directly on the diamond surface after treating oxidation and fluorination for detection of DNAs, respectively. For simple process, immobilization of probe DNAs was carried out directly on the partially aminated diamond without linker molecules. After fabricating micropattemed diamond, specific hybridization with Cy-5 labeled target DNA at a concentration of 100 nM could be clearly detected on H-terminated, partially O-terminated, and partially F-terminated diamonds, respectively. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.

    AB - Amino groups were functionalized directly on the diamond surface after treating oxidation and fluorination for detection of DNAs, respectively. For simple process, immobilization of probe DNAs was carried out directly on the partially aminated diamond without linker molecules. After fabricating micropattemed diamond, specific hybridization with Cy-5 labeled target DNA at a concentration of 100 nM could be clearly detected on H-terminated, partially O-terminated, and partially F-terminated diamonds, respectively. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.

    UR - http://www.scopus.com/inward/record.url?scp=40949163548&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=40949163548&partnerID=8YFLogxK

    M3 - Conference contribution

    AN - SCOPUS:40949163548

    SN - 9781604234060

    VL - 950

    SP - 220

    EP - 227

    BT - Materials Research Society Symposium Proceedings

    ER -