Direct magnetic separation of immune cells from whole blood using bacterial magnetic particles displaying protein G

Masayuki Takahashi, Tomoko Yoshino, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Direct separation of target cells from mixed population, such as peripheral blood, umbilical cord blood, and bone marrow, is an essential technique for various therapeutic or diagnosis applications. In this study, novel particles were fabricated, and direct magnetic separation of immune cells from whole blood using such particles was performed. The mag- netotactic bacterium Magnetospirillum magneticum AMB-1 synthesizes intracellular bacterial magnetic particles (BacMPs), and protein G was expressed on the surface of the BacMPs by gene fusion techniques with anchor proteins isolated from BacMP membrane. The BacMPs displaying protein G (protein G-BacMPs) had high binding capabilities to a wide range of antibody types, and various versions of protein G-BacMPs binding with different anti-CD monoclonal antibodies were constructed. Consequently, direct magnetic separation of immune cells from whole blood using protein G-BacMPs binding with anti-CD monoclonal antibodies was demonstrated. B lymphocytes (CD19+ cells) or T lymphocytes (CD3+ cells), which represent less than 0.05% in whole blood cells, were successfully separated at a purity level of more than 96%. This level was superior to that from previous reports using other magnetic separation approaches. The results of this study demonstrate the utility of protein G-BacMP and this particle may become a powerful tool for various therapeutic or diagnosis applications.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalBiotechnology Progress
Volume25
Issue number1
DOIs
Publication statusPublished - 2009 Jan
Externally publishedYes

Fingerprint

Cell Separation
Blood Cells
Magnetospirillum
Proteins
Monoclonal Antibodies
Bacterial Proteins
Gene Fusion
Fetal Blood
GTP-Binding Proteins
Blood Proteins
B-Lymphocytes
Bone Marrow
Bacteria
T-Lymphocytes
Membranes
Antibodies
Therapeutics
Population

Keywords

  • Bacterial magnetic particle
  • Immune cell
  • Magnetic cell separation
  • Protein display
  • Whole blood

ASJC Scopus subject areas

  • Biotechnology

Cite this

Direct magnetic separation of immune cells from whole blood using bacterial magnetic particles displaying protein G. / Takahashi, Masayuki; Yoshino, Tomoko; Takeyama, Haruko; Matsunaga, Tadashi.

In: Biotechnology Progress, Vol. 25, No. 1, 01.2009, p. 219-226.

Research output: Contribution to journalArticle

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