Direct measurement of local dissolved oxygen concentration spatial profiles in a cell culture environment

Yuki Kagawa, Katsuhisa Matsuura, Tatsuya Shimizu, Satoshi Tsuneda

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Controlling local dissolved oxygen concentration (DO) in media is critical for cell or tissue cultures. Various biomaterials and culture methods have been developed to modulate DO. Direct measurement of local DO in cultures has not been validated as a method to test DO modulation. In the present study we developed a DO measurement system equipped with a Clark-type oxygen microelectrode manipulated with 1μm precision in three-dimensional space to explore potential applications for tissue engineering. By determining the microelectrode tip position precisely against the bottom plane of culture dishes with rat or human cardiac cells in static monolayer culture, we successfully obtained spatial distributions of DO in the medium. Theoretical quantitative predictions fit the obtained data well. Based on analyses of the variance between samples, we found the data reflected "local" oxygen consumption in the vicinity of the microelectrode and the detection of temporal changes in oxygen consumption rates of cultured cells was limited by the diffusion rate of oxygen in the medium. This oxygen measuring system monitors local oxygen consumption and production with high spatial resolution, and can potentially be used with recently developed oxygen modulating biomaterials to design microenvironments and non-invasively monitor local DO dynamics during culture.

    Original languageEnglish
    Pages (from-to)1263-1274
    Number of pages12
    JournalBiotechnology and Bioengineering
    Volume112
    Issue number6
    DOIs
    Publication statusPublished - 2015 Jun 1

    Fingerprint

    Dissolved oxygen
    Cell culture
    Cell Culture Techniques
    Oxygen
    Microelectrodes
    Biocompatible Materials
    Biomaterials
    Oxygen Consumption
    Tissue culture
    Bioelectric potentials
    Tissue engineering
    Spatial distribution
    Rats
    Monolayers
    Cells
    Modulation
    Tissue Engineering
    Cultured Cells
    Analysis of Variance

    Keywords

    • Cardiomyocyte
    • Cell metabolism
    • Oxygen consumption
    • Oxygen microsensor

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

    Cite this

    Direct measurement of local dissolved oxygen concentration spatial profiles in a cell culture environment. / Kagawa, Yuki; Matsuura, Katsuhisa; Shimizu, Tatsuya; Tsuneda, Satoshi.

    In: Biotechnology and Bioengineering, Vol. 112, No. 6, 01.06.2015, p. 1263-1274.

    Research output: Contribution to journalArticle

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