Direct observation of DNA rotation during transcription by escherichia coli RNA polymerase

Yoshie Harada, Osamu Ohara, Akira Takatsuki, Hiroyasu Itoh, Nobuo Shimamoto, Kazuhiko Kinosita

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

Helical filaments driven by linear molecular motors are anticipated to rotate around their axis, but rotation consistent with the helical pitch has not been observed. 14S dynein1 and non-claret disjunctional protein (ncd)2 rotated a microtubule more efficiently than expected for its helical pitch, and myosin rotated an actin filament only poorly3. For DNA-based motors such as RNA polymerase, transcription-induced supercoiling of DNA4 supports the general picture of tracking along the DNA helix5. Here we report direct and real-time optical microscopy measurements of rotation rate that are consistent with high-fidelity tracking. Single RNA polymerase molecules attached to a glass surface rotated DNA for >100 revolutions around the right-handed screw axis of the double helix with a rotary torque of >5 pNnm. This real-time observation of rotation opens the possibility of resolving individual transcription steps.

Original languageEnglish
Pages (from-to)113-115
Number of pages3
JournalNature
Volume409
Issue number6816
DOIs
Publication statusPublished - 2001 Jan 4
Externally publishedYes

Fingerprint

DNA-Directed RNA Polymerases
Observation
Escherichia coli
DNA
Torque
Myosins
Actin Cytoskeleton
Microtubules
Glass
Microscopy
Proteins

ASJC Scopus subject areas

  • General

Cite this

Harada, Y., Ohara, O., Takatsuki, A., Itoh, H., Shimamoto, N., & Kinosita, K. (2001). Direct observation of DNA rotation during transcription by escherichia coli RNA polymerase. Nature, 409(6816), 113-115. https://doi.org/10.1038/35051126

Direct observation of DNA rotation during transcription by escherichia coli RNA polymerase. / Harada, Yoshie; Ohara, Osamu; Takatsuki, Akira; Itoh, Hiroyasu; Shimamoto, Nobuo; Kinosita, Kazuhiko.

In: Nature, Vol. 409, No. 6816, 04.01.2001, p. 113-115.

Research output: Contribution to journalArticle

Harada, Y, Ohara, O, Takatsuki, A, Itoh, H, Shimamoto, N & Kinosita, K 2001, 'Direct observation of DNA rotation during transcription by escherichia coli RNA polymerase', Nature, vol. 409, no. 6816, pp. 113-115. https://doi.org/10.1038/35051126
Harada Y, Ohara O, Takatsuki A, Itoh H, Shimamoto N, Kinosita K. Direct observation of DNA rotation during transcription by escherichia coli RNA polymerase. Nature. 2001 Jan 4;409(6816):113-115. https://doi.org/10.1038/35051126
Harada, Yoshie ; Ohara, Osamu ; Takatsuki, Akira ; Itoh, Hiroyasu ; Shimamoto, Nobuo ; Kinosita, Kazuhiko. / Direct observation of DNA rotation during transcription by escherichia coli RNA polymerase. In: Nature. 2001 ; Vol. 409, No. 6816. pp. 113-115.
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