Direct observation of the myosin va recovery stroke that contributes to unidirectional stepping along actin

Katsuyuki Shiroguchi, Harvey F. Chin, Diane E. Hannemann, Eiro Muneyuki, Enrique M. de la Cruz, Kazuhiko Kinosita

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Myosins are ATP-driven linear molecular motors that work as cellular force generators, transporters, and force sensors. These functions are driven by large-scale nucleotide-dependent conformational changes, termed "strokes"; the "power stroke" is the force-generating swinging of the myosin light chain-binding "neck" domain relative to the motor domain "head" while bound to actin; the "recovery stroke" is the necessary initial motion that primes, or "cocks," myosin while detached from actin. Myosin Va is a processive dimer that steps unidirectionally along actin following a "hand over hand" mechanism in which the trailing head detaches and steps forward ~72 nm. Despite large rotational Brownian motion of the detached head about a free joint adjoining the two necks, unidirectional stepping is achieved, in part by the power stroke of the attached head that moves the joint forward. However, the power stroke alone cannot fully account for preferential forward site binding since the orientation and angle stability of the detached head, which is determined by the properties of the recovery stroke, dictate actin binding site accessibility. Here, we directly observe the recovery stroke dynamics and fluctuations of myosin Va using a novel, transient caged ATP-controlling system that maintains constant ATP levels through stepwise UV-pulse sequences of varying intensity. We immobilized the neck of monomeric myosin Va on a surface and observed real time motions of bead(s) attached site-specifically to the head. ATP induces a transient swing of the neck to the post-recovery stroke conformation, where it remains for ∼40 s, until ATP hydrolysis products are released. Angle distributions indicate that the post-recovery stroke conformation is stabilized by ≥5 kBT of energy. The high kinetic and energetic stability of the post-recovery stroke conformation favors preferential binding of the detached head to a forward site 72 nm away. Thus, the recovery stroke contributes to unidirectional stepping of myosin Va.

    Original languageEnglish
    Article numbere1001031
    JournalPLoS Biology
    Volume9
    Issue number4
    DOIs
    Publication statusPublished - 2011 Apr

    Fingerprint

    Myosins
    myosin
    stroke
    actin
    Actins
    Stroke
    Observation
    Recovery
    Head
    Adenosine Triphosphate
    Conformations
    neck
    Neck
    Binding Sites
    Myosin Light Chains
    binding sites
    Brownian movement
    hands
    Hand
    Joints

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Immunology and Microbiology(all)
    • Neuroscience(all)

    Cite this

    Shiroguchi, K., Chin, H. F., Hannemann, D. E., Muneyuki, E., de la Cruz, E. M., & Kinosita, K. (2011). Direct observation of the myosin va recovery stroke that contributes to unidirectional stepping along actin. PLoS Biology, 9(4), [e1001031]. https://doi.org/10.1371/journal.pbio.1001031

    Direct observation of the myosin va recovery stroke that contributes to unidirectional stepping along actin. / Shiroguchi, Katsuyuki; Chin, Harvey F.; Hannemann, Diane E.; Muneyuki, Eiro; de la Cruz, Enrique M.; Kinosita, Kazuhiko.

    In: PLoS Biology, Vol. 9, No. 4, e1001031, 04.2011.

    Research output: Contribution to journalArticle

    Shiroguchi, K, Chin, HF, Hannemann, DE, Muneyuki, E, de la Cruz, EM & Kinosita, K 2011, 'Direct observation of the myosin va recovery stroke that contributes to unidirectional stepping along actin', PLoS Biology, vol. 9, no. 4, e1001031. https://doi.org/10.1371/journal.pbio.1001031
    Shiroguchi, Katsuyuki ; Chin, Harvey F. ; Hannemann, Diane E. ; Muneyuki, Eiro ; de la Cruz, Enrique M. ; Kinosita, Kazuhiko. / Direct observation of the myosin va recovery stroke that contributes to unidirectional stepping along actin. In: PLoS Biology. 2011 ; Vol. 9, No. 4.
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