Direct observation of the rotation of ε subunit in F1-ATPase

Yasuyuki Kato-Yamada, Hiroyuki Noji, Ryohei Yasuda, Kazuhiko Kinosita, Masasuke Yoshida

Research output: Contribution to journalArticle

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Abstract

Rotation of the ε subunit in F1-ATPase from thermophilic Bacillus strain PS3 (TF1) was observed under a fluorescence microscope by the method used for observation of the γ subunit rotation (Noji, H., Yasuda, R., Yoshida, M., and Kinosita, K., Jr. (1997) Nature 386, 299-302). The α3β3γε complex of TF1 was fixed to a solid surface, and fluorescently labeled actin filament was attached to the ε subunit through biotin- streptavidin. In the presence of ATP, the filament attached to ε subunit rotated in a unidirection. The direction of the rotation was the same as that observed for the γ subunit. The rotational velocity was slightly slower than the filament attached to the γ subunit, probably due to the experimental setup used. Thus, as suggested from biochemical studies (Aggeler, R., Ogilvie, I., and Capaldi, R. A. (1997) J. Biol. Chem. 272, 19621-19624), the ε subunit rotates with the γ subunit in F1-ATPase during catalysis.

Original languageEnglish
Pages (from-to)19375-19377
Number of pages3
JournalJournal of Biological Chemistry
Volume273
Issue number31
DOIs
Publication statusPublished - 1998 Jul 31
Externally publishedYes

Fingerprint

Proton-Translocating ATPases
Observation
Streptavidin
Bacilli
Biotin
Catalysis
Actin Cytoskeleton
Bacillus
Actins
Microscopes
Adenosine Triphosphate
Fluorescence

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kato-Yamada, Y., Noji, H., Yasuda, R., Kinosita, K., & Yoshida, M. (1998). Direct observation of the rotation of ε subunit in F1-ATPase. Journal of Biological Chemistry, 273(31), 19375-19377. https://doi.org/10.1074/jbc.273.31.19375

Direct observation of the rotation of ε subunit in F1-ATPase. / Kato-Yamada, Yasuyuki; Noji, Hiroyuki; Yasuda, Ryohei; Kinosita, Kazuhiko; Yoshida, Masasuke.

In: Journal of Biological Chemistry, Vol. 273, No. 31, 31.07.1998, p. 19375-19377.

Research output: Contribution to journalArticle

Kato-Yamada, Y, Noji, H, Yasuda, R, Kinosita, K & Yoshida, M 1998, 'Direct observation of the rotation of ε subunit in F1-ATPase', Journal of Biological Chemistry, vol. 273, no. 31, pp. 19375-19377. https://doi.org/10.1074/jbc.273.31.19375
Kato-Yamada Y, Noji H, Yasuda R, Kinosita K, Yoshida M. Direct observation of the rotation of ε subunit in F1-ATPase. Journal of Biological Chemistry. 1998 Jul 31;273(31):19375-19377. https://doi.org/10.1074/jbc.273.31.19375
Kato-Yamada, Yasuyuki ; Noji, Hiroyuki ; Yasuda, Ryohei ; Kinosita, Kazuhiko ; Yoshida, Masasuke. / Direct observation of the rotation of ε subunit in F1-ATPase. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 31. pp. 19375-19377.
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