Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

Hideki Itoh, Satoshi Arai, Thankiah Sudhaharan, Sung Chan Lee, Young Tae Chang, Shin'ichi Ishiwata, Madoka Suzuki, E. Birgitte Lane

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca2+ burst.

Original languageEnglish
Pages (from-to)4458-4461
Number of pages4
JournalChemical Communications
Volume52
Issue number24
DOIs
Publication statusPublished - 2016

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Microscopic examination
Fluorescence
Imaging techniques
Coloring Agents
Dyes
Molecules
Temperature
Hot Temperature

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Metals and Alloys

Cite this

Itoh, H., Arai, S., Sudhaharan, T., Lee, S. C., Chang, Y. T., Ishiwata, S., ... Lane, E. B. (2016). Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes. Chemical Communications, 52(24), 4458-4461. https://doi.org/10.1039/c5cc09943a

Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes. / Itoh, Hideki; Arai, Satoshi; Sudhaharan, Thankiah; Lee, Sung Chan; Chang, Young Tae; Ishiwata, Shin'ichi; Suzuki, Madoka; Lane, E. Birgitte.

In: Chemical Communications, Vol. 52, No. 24, 2016, p. 4458-4461.

Research output: Contribution to journalArticle

Itoh, H, Arai, S, Sudhaharan, T, Lee, SC, Chang, YT, Ishiwata, S, Suzuki, M & Lane, EB 2016, 'Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes', Chemical Communications, vol. 52, no. 24, pp. 4458-4461. https://doi.org/10.1039/c5cc09943a
Itoh, Hideki ; Arai, Satoshi ; Sudhaharan, Thankiah ; Lee, Sung Chan ; Chang, Young Tae ; Ishiwata, Shin'ichi ; Suzuki, Madoka ; Lane, E. Birgitte. / Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes. In: Chemical Communications. 2016 ; Vol. 52, No. 24. pp. 4458-4461.
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