Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

Hideki Itoh; Satoshi Arai; Thankiah Sudhaharan; Sung Chan Lee; Young Tae Chang; Shin'ichi Ishiwata; Madoka Suzuki; E. Birgitte Lane

doi:10.1039/c5cc09943a

Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

Hideki Itoh, Satoshi Arai, Thankiah Sudhaharan, Sung Chan Lee, Young Tae Chang, Shin'ichi Ishiwata^*, Madoka Suzuki, E. Birgitte Lane

^*Corresponding author for this work

Waseda Research Institute for Science and Engineering

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca²⁺ burst.

Original language	English
Pages (from-to)	4458-4461
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	24
DOIs	https://doi.org/10.1039/c5cc09943a
Publication status	Published - 2016

ASJC Scopus subject areas

Chemistry(all)
Catalysis
Ceramics and Composites
Electronic, Optical and Magnetic Materials
Surfaces, Coatings and Films
Materials Chemistry
Metals and Alloys

Access to Document

10.1039/c5cc09943a

Cite this

@article{1122bd3265a249d5817c5afa133e3d3f,

title = "Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes",

abstract = "We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca2+ burst.",

author = "Hideki Itoh and Satoshi Arai and Thankiah Sudhaharan and Lee, {Sung Chan} and Chang, {Young Tae} and Shin'ichi Ishiwata and Madoka Suzuki and Lane, {E. Birgitte}",

year = "2016",

doi = "10.1039/c5cc09943a",

language = "English",

volume = "52",

pages = "4458--4461",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "24",

}

TY - JOUR

T1 - Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

AU - Itoh, Hideki

AU - Arai, Satoshi

AU - Sudhaharan, Thankiah

AU - Lee, Sung Chan

AU - Chang, Young Tae

AU - Ishiwata, Shin'ichi

AU - Suzuki, Madoka

AU - Lane, E. Birgitte

PY - 2016

Y1 - 2016

N2 - We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca2+ burst.

AB - We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca2+ burst.

UR - http://www.scopus.com/inward/record.url?scp=84962612478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962612478&partnerID=8YFLogxK

U2 - 10.1039/c5cc09943a

DO - 10.1039/c5cc09943a

M3 - Article

AN - SCOPUS:84962612478

SN - 1359-7345

VL - 52

SP - 4458

EP - 4461

JO - Chemical Communications

JF - Chemical Communications

IS - 24

ER -

Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this