Direct Production of Citric Acid from Starch by a 2-DeoxyglucoseResistant Mutant Strain of Aspergillus niger

Atsushi Suzuki, Somsak Sarangbin, Kotaro Kirimura, Shoji Usami

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    Citric acid production from starch by Aspergillus niger was studied by the shake and semi-solid culture methods. For citric acid production, the parental strain Yang no. 2 and a 2-deoxyglucose (DG)-resistant mutant strain, C192, were used. When cultivated in shake culture with 140 g/l soluble starch as a carbon source, strain C192 produced 69.5 g/l of citric acid, 1.54 times that produced by Yang no. 2, and showed enhanced glucoamylase production (a maximum level of 0.134 μkat/ml at 5 d). From a practical viewpoint, direct production of citric acid from corn- and potato starch was examined using the semi-solid culture method. When cultivated in a semi-solid culture using bagasse as a carrier, C192 produced 107.4 and 92.9 g/l of citric acid, approximately 1.14 and 1.09 times as much as Yang no. 2, from 200 g/l of corn- and potato starch, respectively. C192 exhibited enhanced glucoamylase production during the entire cultivation periods in comparison with that of Yang no. 2.

    Original languageEnglish
    Pages (from-to)320-323
    Number of pages4
    JournalJournal of Fermentation and Bioengineering
    Volume81
    Issue number4
    Publication statusPublished - 1996

    Fingerprint

    Aspergillus niger
    Aspergillus
    Citric acid
    Starch
    Citric Acid
    Glucan 1,4-alpha-Glucosidase
    Solanum tuberosum
    Zea mays
    Bagasse
    Deoxyglucose
    Carbon

    Keywords

    • Aspergillus niger
    • Citric acid
    • Deoxyglucose-resistant mutant
    • Glucoamylase
    • Starch

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

    Cite this

    Direct Production of Citric Acid from Starch by a 2-DeoxyglucoseResistant Mutant Strain of Aspergillus niger. / Suzuki, Atsushi; Sarangbin, Somsak; Kirimura, Kotaro; Usami, Shoji.

    In: Journal of Fermentation and Bioengineering, Vol. 81, No. 4, 1996, p. 320-323.

    Research output: Contribution to journalArticle

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    AU - Usami, Shoji

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