Direct Profiling of rRNA in Saline Wastewater Treatment Samples Using an Oligonucleotide Microarray

Hidetoshi Urakawa; Junpei Matsumoto; Tatsuhiko Hoshino; Satoshi Tsuneda

doi:10.1264/jsme2.22.116

Direct Profiling of rRNA in Saline Wastewater Treatment Samples Using an Oligonucleotide Microarray

Hidetoshi Urakawa^*, Junpei Matsumoto, Tatsuhiko Hoshino, Satoshi Tsuneda

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Fifteen oligonucleotide probes, targeting a broad range of microorganisms, were spotted on a DNA microarray. Specificity and reproducibility were evaluated using fluorescently labeled rRNA from 16 bacterial and eukaryal strains. The oligonucleotide microarray was then used to analyze activated sludge samples of saline industrial wastewater in which microbial communities had been characterized previously using 16S rRNA clone libraries. The results obtained were partially consistent with the results of cloning, and demonstrate the possibility of using oligonucleotide microarrays for the monitoring and characterization of saline industrial wastewater populations.

Original language	English
Pages (from-to)	116-122
Number of pages	7
Journal	Microbes and Environments
Volume	22
Issue number	2
DOIs	https://doi.org/10.1264/jsme2.22.116
Publication status	Published - 2007

Keywords

oligonucleotide microarray
ribosomal RNA
saline industrial wastewater

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Soil Science
Plant Science

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10.1264/jsme2.22.116

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abstract = "Fifteen oligonucleotide probes, targeting a broad range of microorganisms, were spotted on a DNA microarray. Specificity and reproducibility were evaluated using fluorescently labeled rRNA from 16 bacterial and eukaryal strains. The oligonucleotide microarray was then used to analyze activated sludge samples of saline industrial wastewater in which microbial communities had been characterized previously using 16S rRNA clone libraries. The results obtained were partially consistent with the results of cloning, and demonstrate the possibility of using oligonucleotide microarrays for the monitoring and characterization of saline industrial wastewater populations.",

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AU - Hoshino, Tatsuhiko

AU - Tsuneda, Satoshi

PY - 2007

Y1 - 2007

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AB - Fifteen oligonucleotide probes, targeting a broad range of microorganisms, were spotted on a DNA microarray. Specificity and reproducibility were evaluated using fluorescently labeled rRNA from 16 bacterial and eukaryal strains. The oligonucleotide microarray was then used to analyze activated sludge samples of saline industrial wastewater in which microbial communities had been characterized previously using 16S rRNA clone libraries. The results obtained were partially consistent with the results of cloning, and demonstrate the possibility of using oligonucleotide microarrays for the monitoring and characterization of saline industrial wastewater populations.

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KW - ribosomal RNA

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Direct Profiling of rRNA in Saline Wastewater Treatment Samples Using an Oligonucleotide Microarray

Abstract

Keywords

ASJC Scopus subject areas

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