Direct Profiling of rRNA in Saline Wastewater Treatment Samples Using an Oligonucleotide Microarray

Hidetoshi Urakawa, Junpei Matsumoto, Tatsuhiko Hoshino, Satoshi Tsuneda

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Fifteen oligonucleotide probes, targeting a broad range of microorganisms, were spotted on a DNA microarray. Specificity and reproducibility were evaluated using fluorescently labeled rRNA from 16 bacterial and eukaryal strains. The oligonucleotide microarray was then used to analyze activated sludge samples of saline industrial wastewater in which microbial communities had been characterized previously using 16S rRNA clone libraries. The results obtained were partially consistent with the results of cloning, and demonstrate the possibility of using oligonucleotide microarrays for the monitoring and characterization of saline industrial wastewater populations.

    Original languageEnglish
    Pages (from-to)116-122
    Number of pages7
    JournalMicrobes and Environments
    Volume22
    Issue number2
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    oligonucleotides
    wastewater treatment
    wastewater
    ribosomal RNA
    oligonucleotide probes
    activated sludge
    reproducibility
    targeting
    microbial communities
    microbial community
    clone
    molecular cloning
    microorganism
    probe
    clones
    microorganisms
    DNA
    sampling
    monitoring
    DNA microarrays

    Keywords

    • oligonucleotide microarray
    • ribosomal RNA
    • saline industrial wastewater

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Soil Science
    • Plant Science

    Cite this

    Direct Profiling of rRNA in Saline Wastewater Treatment Samples Using an Oligonucleotide Microarray. / Urakawa, Hidetoshi; Matsumoto, Junpei; Hoshino, Tatsuhiko; Tsuneda, Satoshi.

    In: Microbes and Environments, Vol. 22, No. 2, 2007, p. 116-122.

    Research output: Contribution to journalArticle

    Urakawa, Hidetoshi ; Matsumoto, Junpei ; Hoshino, Tatsuhiko ; Tsuneda, Satoshi. / Direct Profiling of rRNA in Saline Wastewater Treatment Samples Using an Oligonucleotide Microarray. In: Microbes and Environments. 2007 ; Vol. 22, No. 2. pp. 116-122.
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