Direct uptake and degradation of DNA by lysosomes

Yuuki Fujiwara, Hisae Kikuchi, Shu Aizawa, Akiko Furuta, Yusuke Hatanaka, Chiho Konya, Kenko Uchida, Keiji Wada, Tomohiro Kabuta

    Research output: Contribution to journalArticle

    44 Citations (Scopus)

    Abstract

    Lysosomes contain various hydrolases that can degrade proteins, lipids, nucleic acids and carbohydrates. We recently discovered "RN autophagy," an autophagic pathway in which RN A is directly taken up by lysosomes and degraded. A lysosomal membrane protein, LAMP2C, a splice variant of LAMP2, binds to RN A and acts as a receptor for this pathway. In the present study, we show that DN A is also directly taken up by lysosomes and degraded. Like RN autophagy, this autophagic pathway, which we term "DN autophagy," is dependent on ATP. The cytosolic sequence of LAMP2C also directly interacts with DN A, and LAMP2C functions as a receptor for DN autophagy, in addition to RN autophagy. Similarly to RN A, DN A binds to the cytosolic sequences of fly and nematode LAMP orthologs. Together with the findings of our previous study, our present findings suggest that RN autophagy and DN autophagy are evolutionarily conserved systems in Metazoa.

    Original languageEnglish
    Pages (from-to)1167-1171
    Number of pages5
    JournalAutophagy
    Volume9
    Issue number8
    DOIs
    Publication statusPublished - 2013 Aug

    Fingerprint

    Autophagy
    Lysosomes
    DNA
    Lysosome-Associated Membrane Glycoproteins
    Hydrolases
    Diptera
    Nucleic Acids
    Adenosine Triphosphate
    Carbohydrates
    Lipids
    Proteins

    Keywords

    • Autophagy
    • DNA
    • DNautophagy
    • LAMP-2
    • LAMP-2C
    • LAMP2
    • LAMP2C
    • RNA
    • RNautophagy

    ASJC Scopus subject areas

    • Cell Biology
    • Molecular Biology

    Cite this

    Fujiwara, Y., Kikuchi, H., Aizawa, S., Furuta, A., Hatanaka, Y., Konya, C., ... Kabuta, T. (2013). Direct uptake and degradation of DNA by lysosomes. Autophagy, 9(8), 1167-1171. https://doi.org/10.4161/auto.24880

    Direct uptake and degradation of DNA by lysosomes. / Fujiwara, Yuuki; Kikuchi, Hisae; Aizawa, Shu; Furuta, Akiko; Hatanaka, Yusuke; Konya, Chiho; Uchida, Kenko; Wada, Keiji; Kabuta, Tomohiro.

    In: Autophagy, Vol. 9, No. 8, 08.2013, p. 1167-1171.

    Research output: Contribution to journalArticle

    Fujiwara, Y, Kikuchi, H, Aizawa, S, Furuta, A, Hatanaka, Y, Konya, C, Uchida, K, Wada, K & Kabuta, T 2013, 'Direct uptake and degradation of DNA by lysosomes', Autophagy, vol. 9, no. 8, pp. 1167-1171. https://doi.org/10.4161/auto.24880
    Fujiwara Y, Kikuchi H, Aizawa S, Furuta A, Hatanaka Y, Konya C et al. Direct uptake and degradation of DNA by lysosomes. Autophagy. 2013 Aug;9(8):1167-1171. https://doi.org/10.4161/auto.24880
    Fujiwara, Yuuki ; Kikuchi, Hisae ; Aizawa, Shu ; Furuta, Akiko ; Hatanaka, Yusuke ; Konya, Chiho ; Uchida, Kenko ; Wada, Keiji ; Kabuta, Tomohiro. / Direct uptake and degradation of DNA by lysosomes. In: Autophagy. 2013 ; Vol. 9, No. 8. pp. 1167-1171.
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