Directed evolution of carotenoid synthases for the production of unnatural carotenoids

Maiko Furubayashi, Daisuke Umeno

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Directed evolution is a well-established strategy to confer novel catalytic functions to the enzymes. Thanks to the relative ease of establishing color screening, carotenogenic enzymes can be rapidly evolved in the laboratory for novel functions. The combinatorial usages of the evolvants result in the creation of diverse set of novel, sometimes unnatural carotenoids. This chapter describes the directed evolution of diapophytoene (C30 carotenoid) synthase CrtM to function in the foreign C40 pathway, and the use of the CrtM variants thus obtained for the production of novel backbone structures.

Original languageEnglish
Title of host publicationMicrobial Carotenoids from Bacteria and Microalgae
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages245-253
Number of pages9
ISBN (Print)9781617798788
DOIs
Publication statusPublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume892
ISSN (Print)1064-3745

Keywords

  • Color screening
  • Combinatorial biosynthesis
  • Directed evolution
  • Error-prone PCR
  • Library construction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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