Disassembly from both ends of thick filaments in rabbit skeletal muscle fibers. An optical diffraction study

S. Ishiwata, K. Muramatsu, H. Higuchi

    Research output: Contribution to journalArticle

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    Abstract

    We show in this paper that the change of the internal structure of a sarcomere in a rabbit glycerinated psoas muscle fiber can be examined by analyzing the intensity change of the first- and second-order optical diffraction lines. A unit-cell (sarcomere)-structure model has been applied to the estimation of the length of thick filaments in a muscle fiber while they undergo dissociation. The optical factors, except for the unit-cell-structure factor, hardly changed during the dissociation of the filaments. Our results show that thick filaments dissociate from both ends on increasing the KCl concentration in the presence of 10 mM pyrophosphate and 5 mM MgCl2. Micromolar concentrations of Ca2+ suppressed to some extent the dissociation of thick filaments. The disassembly of thick filaments occurred at higher KCl concentrations in the absence of pyrophosphate. There was a correlation between the stability of the thick filament structure and cross-bridge formation, which was induced either by the addition of micromolar concentrations of Ca2+ in the presence of Mg-pyrophosphate or by removal of Mg-pyrophosphate.

    Original languageEnglish
    Pages (from-to)257-266
    Number of pages10
    JournalBiophysical Journal
    Volume47
    Issue number3
    Publication statusPublished - 1985

    Fingerprint

    Sarcomeres
    Skeletal Muscle Fibers
    Psoas Muscles
    Rabbits
    Magnesium Chloride
    Muscles
    magnesium pyrophosphate
    diphosphoric acid

    ASJC Scopus subject areas

    • Biophysics

    Cite this

    Disassembly from both ends of thick filaments in rabbit skeletal muscle fibers. An optical diffraction study. / Ishiwata, S.; Muramatsu, K.; Higuchi, H.

    In: Biophysical Journal, Vol. 47, No. 3, 1985, p. 257-266.

    Research output: Contribution to journalArticle

    Ishiwata, S. ; Muramatsu, K. ; Higuchi, H. / Disassembly from both ends of thick filaments in rabbit skeletal muscle fibers. An optical diffraction study. In: Biophysical Journal. 1985 ; Vol. 47, No. 3. pp. 257-266.
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    N2 - We show in this paper that the change of the internal structure of a sarcomere in a rabbit glycerinated psoas muscle fiber can be examined by analyzing the intensity change of the first- and second-order optical diffraction lines. A unit-cell (sarcomere)-structure model has been applied to the estimation of the length of thick filaments in a muscle fiber while they undergo dissociation. The optical factors, except for the unit-cell-structure factor, hardly changed during the dissociation of the filaments. Our results show that thick filaments dissociate from both ends on increasing the KCl concentration in the presence of 10 mM pyrophosphate and 5 mM MgCl2. Micromolar concentrations of Ca2+ suppressed to some extent the dissociation of thick filaments. The disassembly of thick filaments occurred at higher KCl concentrations in the absence of pyrophosphate. There was a correlation between the stability of the thick filament structure and cross-bridge formation, which was induced either by the addition of micromolar concentrations of Ca2+ in the presence of Mg-pyrophosphate or by removal of Mg-pyrophosphate.

    AB - We show in this paper that the change of the internal structure of a sarcomere in a rabbit glycerinated psoas muscle fiber can be examined by analyzing the intensity change of the first- and second-order optical diffraction lines. A unit-cell (sarcomere)-structure model has been applied to the estimation of the length of thick filaments in a muscle fiber while they undergo dissociation. The optical factors, except for the unit-cell-structure factor, hardly changed during the dissociation of the filaments. Our results show that thick filaments dissociate from both ends on increasing the KCl concentration in the presence of 10 mM pyrophosphate and 5 mM MgCl2. Micromolar concentrations of Ca2+ suppressed to some extent the dissociation of thick filaments. The disassembly of thick filaments occurred at higher KCl concentrations in the absence of pyrophosphate. There was a correlation between the stability of the thick filament structure and cross-bridge formation, which was induced either by the addition of micromolar concentrations of Ca2+ in the presence of Mg-pyrophosphate or by removal of Mg-pyrophosphate.

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