Discovery of a novel avian neurosteroid, 7α-hydroxypregnenolone, and its role in the regulation of the diurnal rhythm of locomotor activity in Japanese quail

Kazuyoshi Tsutsui, Kazuhiko Inoue, Hitomi Miyabara, Saori Suzuki, Yuki Ogura, Yasuko Tobari, Shogo Haraguchi

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    The discovery of two novel avian neurosteroids in the quail brain, 7α- and 7β-hydroxypregnenolone is described. Intracerebroventricular administration of 7α-hydroxypregnenolone, but not 7β-hydroxypregnenolone was found to stimulate locomotor activity of male quail when spontaneous nocturnal activity is low. Diurnal changes in locomotor activity in male quail were found to be correlated with a diurnal change in the concentration of diencephalic 7α-hydroxypregnenolone. This correlation was a not seen in female quail which have a lower amplitude diurnal rhythm of locomotor activity and lower daytime concentrations of diencephalic 7α-hydroxypregnenolone. Treatment of male quail with melatonin was found to depress the synthesis of 7α-hydroxypregnenolone in the diencephalon. This is a previously undescribed role for melatonin in the regulation of neurosteroid synthesis in the brain of any vertebrate. We therefore deduced in male quail, that the nocturnal depression in locomotory activity is a consequence of a depression in diencephalic 7α-hydroxypregnenolone resulting from the inhibitory action of the nocturnal increase in melatonin. This observation may be of widespread significance for the molecular control of rhythmic locomotor activity in all vertebrates.

    Original languageEnglish
    Pages (from-to)117-122
    Number of pages6
    JournalGeneral and Comparative Endocrinology
    Volume163
    Issue number1-2
    DOIs
    Publication statusPublished - 2009 Sep 1

    Fingerprint

    Coturnix
    Locomotion
    Quail
    Circadian Rhythm
    quails
    circadian rhythm
    locomotion
    Neurotransmitter Agents
    melatonin
    Melatonin
    Vertebrates
    vertebrates
    brain
    nocturnal activity
    synthesis
    Diencephalon
    Brain
    7-hydroxypregnenolone
    Coturnix japonica

    Keywords

    • 7α-Hydroxypregnenolone
    • Diurnal changes
    • Locomotor activity
    • Melatonin
    • Neurosteroids
    • Quail brain

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Discovery of a novel avian neurosteroid, 7α-hydroxypregnenolone, and its role in the regulation of the diurnal rhythm of locomotor activity in Japanese quail. / Tsutsui, Kazuyoshi; Inoue, Kazuhiko; Miyabara, Hitomi; Suzuki, Saori; Ogura, Yuki; Tobari, Yasuko; Haraguchi, Shogo.

    In: General and Comparative Endocrinology, Vol. 163, No. 1-2, 01.09.2009, p. 117-122.

    Research output: Contribution to journalArticle

    Tsutsui, Kazuyoshi ; Inoue, Kazuhiko ; Miyabara, Hitomi ; Suzuki, Saori ; Ogura, Yuki ; Tobari, Yasuko ; Haraguchi, Shogo. / Discovery of a novel avian neurosteroid, 7α-hydroxypregnenolone, and its role in the regulation of the diurnal rhythm of locomotor activity in Japanese quail. In: General and Comparative Endocrinology. 2009 ; Vol. 163, No. 1-2. pp. 117-122.
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