Discovery of a pimaricin analog JBIR-13, from Streptomyces bicolor NBRC 12746 as predicted by sequence analysis of type i polyketide synthase gene

Hisayuki Komaki, Miho Izumikawa, Jun Ya Ueda, Takuji Nakashima, Shams Tabrez Khan, Motoki Takagi, Kazuo Shin-Ya

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Sequence analysis of ketosynthase domain amplicons from Streptomyces bicolor NBRC 12746T revealed the presence of previously unreported type I polyketide synthases (PKS-I) genes. The clustering of these genes with the reference PKS-1 sequences suggested the possibility to produce a polyene compound similar to pimaricin. Thus, the cultured sample from NBRC 12746 T was analyzed for the production of polyene compounds. The strain produced an antifungal compound which displayed the UV absorption spectrum of tetraene macrolides. The structure determination based on the spectroscopic analysis of the purified compound resulted in the identification of a novel pimaricin analog JBIR-13 (1). This study therefore strongly suggested that a careful analysis of PKS-I genes can provide valuable information in the search of novel bioactive compounds within a class predicted from phylogenetic analysis.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume83
Issue number1
DOIs
Publication statusPublished - 2009 May
Externally publishedYes

Keywords

  • JBIR-13
  • Pimaricin
  • Polyene macrolide
  • Streptomyces bicolor
  • Type I polyketide synthase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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