Display of Functionally Active PHB Depolymerase on Escherichia Coli Cell Surface

Tomohiro Hiraishi, Koichi Yamashita, Masafumi Sakono, Jun Nakanishi, Liu Tzea Tan, Kumar Sudesh, Hideki Abe, Mizuo Maeda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The display of PHB depolymerase (PhaZ RpiT1 ) from R. pickettii T1 on the surface of E. coli JM109 cells is realized using OprI of P. aeruginosa as the anchoring motif. The fusion protein is stably expressed and its surface localization is verified by immunofluorescence microscopy. The displayed PhaZ RpiT1 retains its cleaving ability for soluble substrates as well as its ability to adsorb to the PHB surface, and also remains catalycically active in the degradation of insoluble polyester materials, in spite of the possible suppression of the enzyme movement on the polymer surface. The results demonstrate that PhaZ RpiT1 -displaying E. coli shows potential for use as a whole-cell biocatalyst for the production of (R)-3-hydroxybutyrate monomers from insoluble PHB materials.

Original languageEnglish
Pages (from-to)218-224
Number of pages7
JournalMacromolecular Bioscience
Volume12
Issue number2
DOIs
Publication statusPublished - 2012 Feb 1
Externally publishedYes

Fingerprint

Escherichia coli
Display devices
Polyesters
3-Hydroxybutyric Acid
Enzymes
Fluorescence Microscopy
Polymers
Biocatalysts
Microscopic examination
Proteins
Fusion reactions
Monomers
Degradation
poly-beta-hydroxybutyrate depolymerase
Substrates

Keywords

  • Bioengineering
  • Cell surface display
  • Degradation
  • PHB depolymerase
  • Poly[(R)-3-hydroxybutyrate]

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Hiraishi, T., Yamashita, K., Sakono, M., Nakanishi, J., Tan, L. T., Sudesh, K., ... Maeda, M. (2012). Display of Functionally Active PHB Depolymerase on Escherichia Coli Cell Surface. Macromolecular Bioscience, 12(2), 218-224. https://doi.org/10.1002/mabi.201100273

Display of Functionally Active PHB Depolymerase on Escherichia Coli Cell Surface. / Hiraishi, Tomohiro; Yamashita, Koichi; Sakono, Masafumi; Nakanishi, Jun; Tan, Liu Tzea; Sudesh, Kumar; Abe, Hideki; Maeda, Mizuo.

In: Macromolecular Bioscience, Vol. 12, No. 2, 01.02.2012, p. 218-224.

Research output: Contribution to journalArticle

Hiraishi, T, Yamashita, K, Sakono, M, Nakanishi, J, Tan, LT, Sudesh, K, Abe, H & Maeda, M 2012, 'Display of Functionally Active PHB Depolymerase on Escherichia Coli Cell Surface', Macromolecular Bioscience, vol. 12, no. 2, pp. 218-224. https://doi.org/10.1002/mabi.201100273
Hiraishi T, Yamashita K, Sakono M, Nakanishi J, Tan LT, Sudesh K et al. Display of Functionally Active PHB Depolymerase on Escherichia Coli Cell Surface. Macromolecular Bioscience. 2012 Feb 1;12(2):218-224. https://doi.org/10.1002/mabi.201100273
Hiraishi, Tomohiro ; Yamashita, Koichi ; Sakono, Masafumi ; Nakanishi, Jun ; Tan, Liu Tzea ; Sudesh, Kumar ; Abe, Hideki ; Maeda, Mizuo. / Display of Functionally Active PHB Depolymerase on Escherichia Coli Cell Surface. In: Macromolecular Bioscience. 2012 ; Vol. 12, No. 2. pp. 218-224.
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