Dissecting the taxonomic heterogeneity within propionibacterium acnes: Proposal for propionibacterium acnes subsp. Acnes subsp. Nov. And propionibacterium acnes subsp. Elongatum subsp. Nov

Itaru Dekio, Renata Culak, Raju Misra, Tom Gaulton, Min Fang, Mitsuo Sakamoto, Moriya Ohkuma, Kenshiro Oshima, Masahira Hattori, Hans Peter Klenk, Dunstan Rajendram, Saheer E. Gharbia, Haroun N. Shah

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Propionibacterium acnes subsp. acnes subsp. Nov. And Propionibacterium acnes subsp. elongatum subsp. Nov. are described. These emanate from the three known phylotypes of P. acnes, designated types I, II and III. Electron microscopy confirmed the filamentous cell shape of type III, showing a striking difference from types I/II, which were short rods. Biochemical tests indicated that, in types I/II, either the pyruvate, L-pyrrolidonyl arylamidase or D-ribose 2 test was positive, whereas all of these were negative among type III strains. Matrix-assisted laserdesorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) spectra, which profile mainly their ribosomal proteins, were different between these two groups. Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) spectra of all phylotypes revealed a specific protein biomarker that was overexpressed in type III strains compared with types I/II only when grown aerobically. Reference strains had high whole-genome similarity between types I (>91 %) and II (>75 %), but a considerably lower level of 72 % similarity with type III. recA and gyrB sequence dendrograms confirmed the distant relatedness of type III, indicating the presence of two distinct centres of variation within the species P. acnes. On the other hand, cellular fatty acid profiles and 16S rRNA gene sequence relatedness (>99.3 %) circumscribed the species. Thus, we propose two subspecies, Propionibacterium acnes subsp. acnes subsp. Nov. for types I/II and Propionibacterium acnes subsp. elongatum subsp. Nov. for type III. The type strain of Propionibacterium acnes subsp. acnes is NCTC 737T (=ATCC 6919T=JCM 6425T=DSM 1897T=CCUG 1794T), while the type strain of Propionibacterium acnes subsp. elongatum is K124T (=NCTC 13655T=JCM 18919T).

Original languageEnglish
Pages (from-to)4776-4787
Number of pages12
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number12
DOIs
Publication statusPublished - 2015 Dec 1
Externally publishedYes

Fingerprint

Propionibacterium acnes
acne
new subspecies
Acne Vulgaris
relatedness
ionization
mass spectrometry
protein
electron microscopy
subspecies
biomarker
desorption
fatty acid
genome
laser
matrix
gene
Mass Spectrometry
Ribose
test

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Dissecting the taxonomic heterogeneity within propionibacterium acnes : Proposal for propionibacterium acnes subsp. Acnes subsp. Nov. And propionibacterium acnes subsp. Elongatum subsp. Nov. / Dekio, Itaru; Culak, Renata; Misra, Raju; Gaulton, Tom; Fang, Min; Sakamoto, Mitsuo; Ohkuma, Moriya; Oshima, Kenshiro; Hattori, Masahira; Klenk, Hans Peter; Rajendram, Dunstan; Gharbia, Saheer E.; Shah, Haroun N.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 12, 01.12.2015, p. 4776-4787.

Research output: Contribution to journalArticle

Dekio, Itaru ; Culak, Renata ; Misra, Raju ; Gaulton, Tom ; Fang, Min ; Sakamoto, Mitsuo ; Ohkuma, Moriya ; Oshima, Kenshiro ; Hattori, Masahira ; Klenk, Hans Peter ; Rajendram, Dunstan ; Gharbia, Saheer E. ; Shah, Haroun N. / Dissecting the taxonomic heterogeneity within propionibacterium acnes : Proposal for propionibacterium acnes subsp. Acnes subsp. Nov. And propionibacterium acnes subsp. Elongatum subsp. Nov. In: International Journal of Systematic and Evolutionary Microbiology. 2015 ; Vol. 65, No. 12. pp. 4776-4787.
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abstract = "Propionibacterium acnes subsp. acnes subsp. Nov. And Propionibacterium acnes subsp. elongatum subsp. Nov. are described. These emanate from the three known phylotypes of P. acnes, designated types I, II and III. Electron microscopy confirmed the filamentous cell shape of type III, showing a striking difference from types I/II, which were short rods. Biochemical tests indicated that, in types I/II, either the pyruvate, L-pyrrolidonyl arylamidase or D-ribose 2 test was positive, whereas all of these were negative among type III strains. Matrix-assisted laserdesorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) spectra, which profile mainly their ribosomal proteins, were different between these two groups. Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) spectra of all phylotypes revealed a specific protein biomarker that was overexpressed in type III strains compared with types I/II only when grown aerobically. Reference strains had high whole-genome similarity between types I (>91 {\%}) and II (>75 {\%}), but a considerably lower level of 72 {\%} similarity with type III. recA and gyrB sequence dendrograms confirmed the distant relatedness of type III, indicating the presence of two distinct centres of variation within the species P. acnes. On the other hand, cellular fatty acid profiles and 16S rRNA gene sequence relatedness (>99.3 {\%}) circumscribed the species. Thus, we propose two subspecies, Propionibacterium acnes subsp. acnes subsp. Nov. for types I/II and Propionibacterium acnes subsp. elongatum subsp. Nov. for type III. The type strain of Propionibacterium acnes subsp. acnes is NCTC 737T (=ATCC 6919T=JCM 6425T=DSM 1897T=CCUG 1794T), while the type strain of Propionibacterium acnes subsp. elongatum is K124T (=NCTC 13655T=JCM 18919T).",
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AU - Dekio, Itaru

AU - Culak, Renata

AU - Misra, Raju

AU - Gaulton, Tom

AU - Fang, Min

AU - Sakamoto, Mitsuo

AU - Ohkuma, Moriya

AU - Oshima, Kenshiro

AU - Hattori, Masahira

AU - Klenk, Hans Peter

AU - Rajendram, Dunstan

AU - Gharbia, Saheer E.

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