Abstract
Propionibacterium acnes subsp. acnes subsp. Nov. And Propionibacterium acnes subsp. elongatum subsp. Nov. are described. These emanate from the three known phylotypes of P. acnes, designated types I, II and III. Electron microscopy confirmed the filamentous cell shape of type III, showing a striking difference from types I/II, which were short rods. Biochemical tests indicated that, in types I/II, either the pyruvate, L-pyrrolidonyl arylamidase or D-ribose 2 test was positive, whereas all of these were negative among type III strains. Matrix-assisted laserdesorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) spectra, which profile mainly their ribosomal proteins, were different between these two groups. Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) spectra of all phylotypes revealed a specific protein biomarker that was overexpressed in type III strains compared with types I/II only when grown aerobically. Reference strains had high whole-genome similarity between types I (>91 %) and II (>75 %), but a considerably lower level of 72 % similarity with type III. recA and gyrB sequence dendrograms confirmed the distant relatedness of type III, indicating the presence of two distinct centres of variation within the species P. acnes. On the other hand, cellular fatty acid profiles and 16S rRNA gene sequence relatedness (>99.3 %) circumscribed the species. Thus, we propose two subspecies, Propionibacterium acnes subsp. acnes subsp. Nov. for types I/II and Propionibacterium acnes subsp. elongatum subsp. Nov. for type III. The type strain of Propionibacterium acnes subsp. acnes is NCTC 737T (=ATCC 6919T=JCM 6425T=DSM 1897T=CCUG 1794T), while the type strain of Propionibacterium acnes subsp. elongatum is K124T (=NCTC 13655T=JCM 18919T).
| Original language | English |
|---|---|
| Pages (from-to) | 4776-4787 |
| Number of pages | 12 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 65 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2015 Dec 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
- Ecology, Evolution, Behavior and Systematics
Cite this
Dissecting the taxonomic heterogeneity within propionibacterium acnes : Proposal for propionibacterium acnes subsp. Acnes subsp. Nov. And propionibacterium acnes subsp. Elongatum subsp. Nov. / Dekio, Itaru; Culak, Renata; Misra, Raju; Gaulton, Tom; Fang, Min; Sakamoto, Mitsuo; Ohkuma, Moriya; Oshima, Kenshiro; Hattori, Masahira; Klenk, Hans Peter; Rajendram, Dunstan; Gharbia, Saheer E.; Shah, Haroun N.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 12, 01.12.2015, p. 4776-4787.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dissecting the taxonomic heterogeneity within propionibacterium acnes
T2 - Proposal for propionibacterium acnes subsp. Acnes subsp. Nov. And propionibacterium acnes subsp. Elongatum subsp. Nov
AU - Dekio, Itaru
AU - Culak, Renata
AU - Misra, Raju
AU - Gaulton, Tom
AU - Fang, Min
AU - Sakamoto, Mitsuo
AU - Ohkuma, Moriya
AU - Oshima, Kenshiro
AU - Hattori, Masahira
AU - Klenk, Hans Peter
AU - Rajendram, Dunstan
AU - Gharbia, Saheer E.
AU - Shah, Haroun N.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Propionibacterium acnes subsp. acnes subsp. Nov. And Propionibacterium acnes subsp. elongatum subsp. Nov. are described. These emanate from the three known phylotypes of P. acnes, designated types I, II and III. Electron microscopy confirmed the filamentous cell shape of type III, showing a striking difference from types I/II, which were short rods. Biochemical tests indicated that, in types I/II, either the pyruvate, L-pyrrolidonyl arylamidase or D-ribose 2 test was positive, whereas all of these were negative among type III strains. Matrix-assisted laserdesorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) spectra, which profile mainly their ribosomal proteins, were different between these two groups. Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) spectra of all phylotypes revealed a specific protein biomarker that was overexpressed in type III strains compared with types I/II only when grown aerobically. Reference strains had high whole-genome similarity between types I (>91 %) and II (>75 %), but a considerably lower level of 72 % similarity with type III. recA and gyrB sequence dendrograms confirmed the distant relatedness of type III, indicating the presence of two distinct centres of variation within the species P. acnes. On the other hand, cellular fatty acid profiles and 16S rRNA gene sequence relatedness (>99.3 %) circumscribed the species. Thus, we propose two subspecies, Propionibacterium acnes subsp. acnes subsp. Nov. for types I/II and Propionibacterium acnes subsp. elongatum subsp. Nov. for type III. The type strain of Propionibacterium acnes subsp. acnes is NCTC 737T (=ATCC 6919T=JCM 6425T=DSM 1897T=CCUG 1794T), while the type strain of Propionibacterium acnes subsp. elongatum is K124T (=NCTC 13655T=JCM 18919T).
AB - Propionibacterium acnes subsp. acnes subsp. Nov. And Propionibacterium acnes subsp. elongatum subsp. Nov. are described. These emanate from the three known phylotypes of P. acnes, designated types I, II and III. Electron microscopy confirmed the filamentous cell shape of type III, showing a striking difference from types I/II, which were short rods. Biochemical tests indicated that, in types I/II, either the pyruvate, L-pyrrolidonyl arylamidase or D-ribose 2 test was positive, whereas all of these were negative among type III strains. Matrix-assisted laserdesorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) spectra, which profile mainly their ribosomal proteins, were different between these two groups. Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) spectra of all phylotypes revealed a specific protein biomarker that was overexpressed in type III strains compared with types I/II only when grown aerobically. Reference strains had high whole-genome similarity between types I (>91 %) and II (>75 %), but a considerably lower level of 72 % similarity with type III. recA and gyrB sequence dendrograms confirmed the distant relatedness of type III, indicating the presence of two distinct centres of variation within the species P. acnes. On the other hand, cellular fatty acid profiles and 16S rRNA gene sequence relatedness (>99.3 %) circumscribed the species. Thus, we propose two subspecies, Propionibacterium acnes subsp. acnes subsp. Nov. for types I/II and Propionibacterium acnes subsp. elongatum subsp. Nov. for type III. The type strain of Propionibacterium acnes subsp. acnes is NCTC 737T (=ATCC 6919T=JCM 6425T=DSM 1897T=CCUG 1794T), while the type strain of Propionibacterium acnes subsp. elongatum is K124T (=NCTC 13655T=JCM 18919T).
UR - http://www.scopus.com/inward/record.url?scp=84955514370&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84955514370&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.000648
DO - 10.1099/ijsem.0.000648
M3 - Article
C2 - 26432704
AN - SCOPUS:84955514370
VL - 65
SP - 4776
EP - 4787
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 12
ER -