Distribution and localization of pituitary adenylate cyclase-activating polypeptide-specific receptor (PAC1R) in the rostral migratory stream of the infant mouse brain

Ryosuke Matsuno, Hirokazu Ohtaki, Tomoya Nakamachi, Jun Watanabe, Sachiko Yofu, Daisuke Hayashi, Takaaki Takeda, Naoko Nonaka, Mayumi Seki, Masahisa Nakamura, Kazuo Itabashi, Seiji Shioda

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Pituitary adenylate cyclase-activating polypeptide (PACAP) is known to participate in the regulation of neuronal proliferation and differentiation. While these processes are considered to be mediated via PACAP's actions on the PACAP-specific receptor, PAC1R, the precise distribution of PAC1R during neurodevelopment has not yet to be elucidated in detail. The purpose of this study is to examine the distribution of PAC1R in the neurogenic region of the rostral migratory stream (RMS) from the apical subventricular zone (SVZa) to the olfactory bulb (OB) in infant mice using immunostaining. Co-immunostaining for PAC1R in a variety types of cell were carried out using different markers. These included the neural stem cell markers, nestin and glial fibrillary acidic protein (GFAP), a marker for migrating neuroblasts (doublecortin, DCX), a marker for immature neurons βIII-tubulin, (Tuj1), and a marker for mature neurons, neuronal nuclei (NeuN). PAC1R-like immunoreactivity (LI) was observed in the RMS. However, the intensity of PAC1R- LI was different depending on the regions which were investigated. PAC1R-LI was strong in nestin- and GFAP-positive cells in the SVZa and was also observed in NeuN-positive cells in the OB. However, the intensities of PAC1R-LI in DCX- and Tuj1-positive cells were weaker than the other markers. These results suggest that PACAP may participate in the neurodevelopment with the stage-specific expression of PAC1R and that PACAP plays important roles in neurons as well as in glial cells.

    Original languageEnglish
    Pages (from-to)80-87
    Number of pages8
    JournalRegulatory Peptides
    Volume145
    Issue number1-3
    DOIs
    Publication statusPublished - 2008 Jan 10

    Fingerprint

    Pituitary Adenylate Cyclase-Activating Polypeptide Receptors
    Pituitary Adenylate Cyclase-Activating Polypeptide
    Brain
    Nestin
    Olfactory Bulb
    Glial Fibrillary Acidic Protein
    Neurons
    Cells
    Neural Stem Cells
    Lateral Ventricles
    Tubulin
    Cell Nucleus
    Neuroglia
    Stem cells

    Keywords

    • Development
    • Doublecortin
    • Mouse
    • Neuroblast
    • Neuronal stem cell
    • PACAP

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Physiology
    • Neuroscience(all)

    Cite this

    Distribution and localization of pituitary adenylate cyclase-activating polypeptide-specific receptor (PAC1R) in the rostral migratory stream of the infant mouse brain. / Matsuno, Ryosuke; Ohtaki, Hirokazu; Nakamachi, Tomoya; Watanabe, Jun; Yofu, Sachiko; Hayashi, Daisuke; Takeda, Takaaki; Nonaka, Naoko; Seki, Mayumi; Nakamura, Masahisa; Itabashi, Kazuo; Shioda, Seiji.

    In: Regulatory Peptides, Vol. 145, No. 1-3, 10.01.2008, p. 80-87.

    Research output: Contribution to journalArticle

    Matsuno, R, Ohtaki, H, Nakamachi, T, Watanabe, J, Yofu, S, Hayashi, D, Takeda, T, Nonaka, N, Seki, M, Nakamura, M, Itabashi, K & Shioda, S 2008, 'Distribution and localization of pituitary adenylate cyclase-activating polypeptide-specific receptor (PAC1R) in the rostral migratory stream of the infant mouse brain', Regulatory Peptides, vol. 145, no. 1-3, pp. 80-87. https://doi.org/10.1016/j.regpep.2007.08.016
    Matsuno, Ryosuke ; Ohtaki, Hirokazu ; Nakamachi, Tomoya ; Watanabe, Jun ; Yofu, Sachiko ; Hayashi, Daisuke ; Takeda, Takaaki ; Nonaka, Naoko ; Seki, Mayumi ; Nakamura, Masahisa ; Itabashi, Kazuo ; Shioda, Seiji. / Distribution and localization of pituitary adenylate cyclase-activating polypeptide-specific receptor (PAC1R) in the rostral migratory stream of the infant mouse brain. In: Regulatory Peptides. 2008 ; Vol. 145, No. 1-3. pp. 80-87.
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    abstract = "Pituitary adenylate cyclase-activating polypeptide (PACAP) is known to participate in the regulation of neuronal proliferation and differentiation. While these processes are considered to be mediated via PACAP's actions on the PACAP-specific receptor, PAC1R, the precise distribution of PAC1R during neurodevelopment has not yet to be elucidated in detail. The purpose of this study is to examine the distribution of PAC1R in the neurogenic region of the rostral migratory stream (RMS) from the apical subventricular zone (SVZa) to the olfactory bulb (OB) in infant mice using immunostaining. Co-immunostaining for PAC1R in a variety types of cell were carried out using different markers. These included the neural stem cell markers, nestin and glial fibrillary acidic protein (GFAP), a marker for migrating neuroblasts (doublecortin, DCX), a marker for immature neurons βIII-tubulin, (Tuj1), and a marker for mature neurons, neuronal nuclei (NeuN). PAC1R-like immunoreactivity (LI) was observed in the RMS. However, the intensity of PAC1R- LI was different depending on the regions which were investigated. PAC1R-LI was strong in nestin- and GFAP-positive cells in the SVZa and was also observed in NeuN-positive cells in the OB. However, the intensities of PAC1R-LI in DCX- and Tuj1-positive cells were weaker than the other markers. These results suggest that PACAP may participate in the neurodevelopment with the stage-specific expression of PAC1R and that PACAP plays important roles in neurons as well as in glial cells.",
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    AU - Nakamachi, Tomoya

    AU - Watanabe, Jun

    AU - Yofu, Sachiko

    AU - Hayashi, Daisuke

    AU - Takeda, Takaaki

    AU - Nonaka, Naoko

    AU - Seki, Mayumi

    AU - Nakamura, Masahisa

    AU - Itabashi, Kazuo

    AU - Shioda, Seiji

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