Distribution of a novel avian gonadotropin-inhibitory hormone in the quail brain

Kazuyoshi Ukena, Takayoshi Ubuka, Kazuyoshi Tsutsui

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

We recently identified a novel hypothalamic neuropeptide inhibiting gonadotropin release in the quail brain and termed it gonadotropin inhibitory hormone (GnIH). In this study, we investigated the localization and distribution of GnIH in both sexes of adult quails by immunohistochemistry with a specific antiserum against GnIH and in situ hybridization. Quantitative analysis demonstrated that the concentration of GnIH in the diencephalon was greater than that in the mesencephalon without sex difference. GnIH concentrations in the cerebrum and cerebellum were below the level of detectability. Clusters of GnIH-like immunoreactive (GnIH-ir) cell bodies were localized in the paraventricular nucleus (PVN) of the hypothalamus. There was no significant difference in the number of GnIH-ir cells in the PVN between males and females. By double immunostaining with antisera reacting with GnIH or avian posterior pituitary hormones (vasotocin and mesotocin), GnIH-ir cells were found to be parvocellular neurons in the ventral portion of PVN, which showed no immunoreaction with the antisera against vasotocin and mesotocin. In situ hybridization revealed the cellular localization of GnIH mRNA in the PVN. GnIH-ir nerve fibers were however widely distributed in the diencephalic and mesencephalic regions. Dense networks of immunoreactive fibers were found in the ventral paleostriatum, septal area, preoptic area, hypothalamus, and optic tectum. The most prominent fibers were seen in the median eminence of the hypothalamus and the dorsal motor nucleus of the vagus in the medulla oblongata. Thus, GnIH may participate not only in neuroendocrine functions, but also in behavioral and autonomic mechanisms.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalCell and Tissue Research
Volume312
Issue number1
Publication statusPublished - 2003 Apr 1
Externally publishedYes

Fingerprint

Quail
Gonadotropins
Brain
Hormones
Paraventricular Hypothalamic Nucleus
Hypothalamus
Vasotocin
Immune Sera
In Situ Hybridization
Fibers
Posterior Pituitary Hormones
Septum of Brain
Diencephalon
Medulla Oblongata
Median Eminence
Globus Pallidus
Preoptic Area
Superior Colliculi
Cerebrum
Mesencephalon

Keywords

  • Brain
  • Enzyme-linked immunosorbent assay
  • Gonadotropin-inhibitory hormone
  • Immunohistochemistry
  • In situ hybridization
  • Japanese quail

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Distribution of a novel avian gonadotropin-inhibitory hormone in the quail brain. / Ukena, Kazuyoshi; Ubuka, Takayoshi; Tsutsui, Kazuyoshi.

In: Cell and Tissue Research, Vol. 312, No. 1, 01.04.2003, p. 73-79.

Research output: Contribution to journalArticle

@article{3d50ba4d3cde486c954df5d6fb5d2201,
title = "Distribution of a novel avian gonadotropin-inhibitory hormone in the quail brain",
abstract = "We recently identified a novel hypothalamic neuropeptide inhibiting gonadotropin release in the quail brain and termed it gonadotropin inhibitory hormone (GnIH). In this study, we investigated the localization and distribution of GnIH in both sexes of adult quails by immunohistochemistry with a specific antiserum against GnIH and in situ hybridization. Quantitative analysis demonstrated that the concentration of GnIH in the diencephalon was greater than that in the mesencephalon without sex difference. GnIH concentrations in the cerebrum and cerebellum were below the level of detectability. Clusters of GnIH-like immunoreactive (GnIH-ir) cell bodies were localized in the paraventricular nucleus (PVN) of the hypothalamus. There was no significant difference in the number of GnIH-ir cells in the PVN between males and females. By double immunostaining with antisera reacting with GnIH or avian posterior pituitary hormones (vasotocin and mesotocin), GnIH-ir cells were found to be parvocellular neurons in the ventral portion of PVN, which showed no immunoreaction with the antisera against vasotocin and mesotocin. In situ hybridization revealed the cellular localization of GnIH mRNA in the PVN. GnIH-ir nerve fibers were however widely distributed in the diencephalic and mesencephalic regions. Dense networks of immunoreactive fibers were found in the ventral paleostriatum, septal area, preoptic area, hypothalamus, and optic tectum. The most prominent fibers were seen in the median eminence of the hypothalamus and the dorsal motor nucleus of the vagus in the medulla oblongata. Thus, GnIH may participate not only in neuroendocrine functions, but also in behavioral and autonomic mechanisms.",
keywords = "Brain, Enzyme-linked immunosorbent assay, Gonadotropin-inhibitory hormone, Immunohistochemistry, In situ hybridization, Japanese quail",
author = "Kazuyoshi Ukena and Takayoshi Ubuka and Kazuyoshi Tsutsui",
year = "2003",
month = "4",
day = "1",
language = "English",
volume = "312",
pages = "73--79",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Distribution of a novel avian gonadotropin-inhibitory hormone in the quail brain

AU - Ukena, Kazuyoshi

AU - Ubuka, Takayoshi

AU - Tsutsui, Kazuyoshi

PY - 2003/4/1

Y1 - 2003/4/1

N2 - We recently identified a novel hypothalamic neuropeptide inhibiting gonadotropin release in the quail brain and termed it gonadotropin inhibitory hormone (GnIH). In this study, we investigated the localization and distribution of GnIH in both sexes of adult quails by immunohistochemistry with a specific antiserum against GnIH and in situ hybridization. Quantitative analysis demonstrated that the concentration of GnIH in the diencephalon was greater than that in the mesencephalon without sex difference. GnIH concentrations in the cerebrum and cerebellum were below the level of detectability. Clusters of GnIH-like immunoreactive (GnIH-ir) cell bodies were localized in the paraventricular nucleus (PVN) of the hypothalamus. There was no significant difference in the number of GnIH-ir cells in the PVN between males and females. By double immunostaining with antisera reacting with GnIH or avian posterior pituitary hormones (vasotocin and mesotocin), GnIH-ir cells were found to be parvocellular neurons in the ventral portion of PVN, which showed no immunoreaction with the antisera against vasotocin and mesotocin. In situ hybridization revealed the cellular localization of GnIH mRNA in the PVN. GnIH-ir nerve fibers were however widely distributed in the diencephalic and mesencephalic regions. Dense networks of immunoreactive fibers were found in the ventral paleostriatum, septal area, preoptic area, hypothalamus, and optic tectum. The most prominent fibers were seen in the median eminence of the hypothalamus and the dorsal motor nucleus of the vagus in the medulla oblongata. Thus, GnIH may participate not only in neuroendocrine functions, but also in behavioral and autonomic mechanisms.

AB - We recently identified a novel hypothalamic neuropeptide inhibiting gonadotropin release in the quail brain and termed it gonadotropin inhibitory hormone (GnIH). In this study, we investigated the localization and distribution of GnIH in both sexes of adult quails by immunohistochemistry with a specific antiserum against GnIH and in situ hybridization. Quantitative analysis demonstrated that the concentration of GnIH in the diencephalon was greater than that in the mesencephalon without sex difference. GnIH concentrations in the cerebrum and cerebellum were below the level of detectability. Clusters of GnIH-like immunoreactive (GnIH-ir) cell bodies were localized in the paraventricular nucleus (PVN) of the hypothalamus. There was no significant difference in the number of GnIH-ir cells in the PVN between males and females. By double immunostaining with antisera reacting with GnIH or avian posterior pituitary hormones (vasotocin and mesotocin), GnIH-ir cells were found to be parvocellular neurons in the ventral portion of PVN, which showed no immunoreaction with the antisera against vasotocin and mesotocin. In situ hybridization revealed the cellular localization of GnIH mRNA in the PVN. GnIH-ir nerve fibers were however widely distributed in the diencephalic and mesencephalic regions. Dense networks of immunoreactive fibers were found in the ventral paleostriatum, septal area, preoptic area, hypothalamus, and optic tectum. The most prominent fibers were seen in the median eminence of the hypothalamus and the dorsal motor nucleus of the vagus in the medulla oblongata. Thus, GnIH may participate not only in neuroendocrine functions, but also in behavioral and autonomic mechanisms.

KW - Brain

KW - Enzyme-linked immunosorbent assay

KW - Gonadotropin-inhibitory hormone

KW - Immunohistochemistry

KW - In situ hybridization

KW - Japanese quail

UR - http://www.scopus.com/inward/record.url?scp=0037570714&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037570714&partnerID=8YFLogxK

M3 - Article

C2 - 12712318

AN - SCOPUS:0037570714

VL - 312

SP - 73

EP - 79

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 1

ER -