Distribution of novel RFamide-related peptide-like immunoreactivity in the mouse central nervous system

Kazuyoshi Ukena, Kazuyoshi Tsutsui

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Recently, novel mammalian RFamide-related peptides (RFRPs) have been identified and suggested to increase prolactin release in the rodent. To assess possible functions of RFRPs, we investigated the distribution of RFRPs in the mouse central nervous system by immunochemical analyses. The quantitative analysis revealed that the concentration of RFRP-like substances was much higher in the brainstem and spinal cord than in other regions. Immunohistochemistry showed that RFRP-like immunoreactive perikarya were localized in the dorsomedial hypothalamic nucleus, the lateral superior olive, and the nucleus of the solitary tract. The dense networks of immunoreactive fibers were found in the lateral parabrachial nucleus, the lateral reticular nucleus, and the superficial layer of spinal trigeminal nucleus and dorsal horn of the spinal cord. Thus, RFRPs may participate not only in neuroendocrine functions but also in behavioral, sensory, and autonomic functions.

Original languageEnglish
Pages (from-to)153-156
Number of pages4
JournalNeuroscience Letters
Volume300
Issue number3
DOIs
Publication statusPublished - 2001 Mar 16
Externally publishedYes

Fingerprint

Central Nervous System
Dorsomedial Hypothalamic Nucleus
Spinal Trigeminal Nucleus
Solitary Nucleus
Prolactin
Brain Stem
RFamide peptide
Rodentia
Spinal Cord
Immunohistochemistry

Keywords

  • Central nervous system
  • Enzyme-linked immunosorbent assay
  • Immunohistochemistry
  • Mouse
  • RFamide-related peptide

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distribution of novel RFamide-related peptide-like immunoreactivity in the mouse central nervous system. / Ukena, Kazuyoshi; Tsutsui, Kazuyoshi.

In: Neuroscience Letters, Vol. 300, No. 3, 16.03.2001, p. 153-156.

Research output: Contribution to journalArticle

@article{911f2fcd71cc4671b537605d6bb06994,
title = "Distribution of novel RFamide-related peptide-like immunoreactivity in the mouse central nervous system",
abstract = "Recently, novel mammalian RFamide-related peptides (RFRPs) have been identified and suggested to increase prolactin release in the rodent. To assess possible functions of RFRPs, we investigated the distribution of RFRPs in the mouse central nervous system by immunochemical analyses. The quantitative analysis revealed that the concentration of RFRP-like substances was much higher in the brainstem and spinal cord than in other regions. Immunohistochemistry showed that RFRP-like immunoreactive perikarya were localized in the dorsomedial hypothalamic nucleus, the lateral superior olive, and the nucleus of the solitary tract. The dense networks of immunoreactive fibers were found in the lateral parabrachial nucleus, the lateral reticular nucleus, and the superficial layer of spinal trigeminal nucleus and dorsal horn of the spinal cord. Thus, RFRPs may participate not only in neuroendocrine functions but also in behavioral, sensory, and autonomic functions.",
keywords = "Central nervous system, Enzyme-linked immunosorbent assay, Immunohistochemistry, Mouse, RFamide-related peptide",
author = "Kazuyoshi Ukena and Kazuyoshi Tsutsui",
year = "2001",
month = "3",
day = "16",
doi = "10.1016/S0304-3940(01)01583-X",
language = "English",
volume = "300",
pages = "153--156",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Distribution of novel RFamide-related peptide-like immunoreactivity in the mouse central nervous system

AU - Ukena, Kazuyoshi

AU - Tsutsui, Kazuyoshi

PY - 2001/3/16

Y1 - 2001/3/16

N2 - Recently, novel mammalian RFamide-related peptides (RFRPs) have been identified and suggested to increase prolactin release in the rodent. To assess possible functions of RFRPs, we investigated the distribution of RFRPs in the mouse central nervous system by immunochemical analyses. The quantitative analysis revealed that the concentration of RFRP-like substances was much higher in the brainstem and spinal cord than in other regions. Immunohistochemistry showed that RFRP-like immunoreactive perikarya were localized in the dorsomedial hypothalamic nucleus, the lateral superior olive, and the nucleus of the solitary tract. The dense networks of immunoreactive fibers were found in the lateral parabrachial nucleus, the lateral reticular nucleus, and the superficial layer of spinal trigeminal nucleus and dorsal horn of the spinal cord. Thus, RFRPs may participate not only in neuroendocrine functions but also in behavioral, sensory, and autonomic functions.

AB - Recently, novel mammalian RFamide-related peptides (RFRPs) have been identified and suggested to increase prolactin release in the rodent. To assess possible functions of RFRPs, we investigated the distribution of RFRPs in the mouse central nervous system by immunochemical analyses. The quantitative analysis revealed that the concentration of RFRP-like substances was much higher in the brainstem and spinal cord than in other regions. Immunohistochemistry showed that RFRP-like immunoreactive perikarya were localized in the dorsomedial hypothalamic nucleus, the lateral superior olive, and the nucleus of the solitary tract. The dense networks of immunoreactive fibers were found in the lateral parabrachial nucleus, the lateral reticular nucleus, and the superficial layer of spinal trigeminal nucleus and dorsal horn of the spinal cord. Thus, RFRPs may participate not only in neuroendocrine functions but also in behavioral, sensory, and autonomic functions.

KW - Central nervous system

KW - Enzyme-linked immunosorbent assay

KW - Immunohistochemistry

KW - Mouse

KW - RFamide-related peptide

UR - http://www.scopus.com/inward/record.url?scp=0035896464&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035896464&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(01)01583-X

DO - 10.1016/S0304-3940(01)01583-X

M3 - Article

VL - 300

SP - 153

EP - 156

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -